Research Keyword: primer design

Development of reverse transcription loop-mediated isothermal amplification-based assay for rapid and specific detection of human fungal pathogen, Candida auris

Researchers developed a new rapid test called RT-LAMP to detect Candida auris, a dangerous multidrug-resistant fungus that causes hospital infections. The test works by changing color from pink to yellow when the fungus is present, making it easy to read without special equipment. It is more sensitive and specific than current methods, can be done quickly at a single temperature, and costs much less than existing tests, making it ideal for hospitals and clinics with limited resources.

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Aspergillus in Italian Pistachios: Characterization and Detection of Major Aflatoxigenic Species With a Loop-Mediated Isothermal Amplification Assay

Researchers studied pistachio nuts from Italy and found dangerous mold species that produce aflatoxins, harmful toxins that can cause serious health problems. They developed a quick and reliable test using molecular biology techniques to detect these molds in pistachios, even at very low levels of contamination. The test uses specially treated dried primers that remain stable for over a month, making it practical for food safety monitoring in warehouses and markets around the world.

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