Research Keyword: phylogenetic identification

Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

King oyster mushrooms are susceptible to green mold caused by various Trichoderma fungi, which significantly reduces crop yields. Researchers developed a quick DNA test that can detect these harmful fungi in mushroom farms before they cause major damage. The test is sensitive enough to identify the fungus even when present in very small amounts mixed with mushroom tissue. This new detection tool will help mushroom farmers monitor their crops and prevent costly contamination losses.

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Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

This study developed a fast molecular test using real-time PCR to detect harmful Aspergillus fungi in grains and seeds. Instead of waiting 3-5 days for traditional culture methods, this new test can identify the fungi in just hours by detecting specific DNA patterns. The test was tested on 140 samples of cereals and oilseeds and proved more accurate than traditional methods, making it useful for food safety in markets and processing plants.

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