What Quality Suffices for Nanopore Metabarcoding? Reconsidering Methodology and Ectomycorrhizae in Decaying Fagus sylvatica Bark as Case Study
This study shows that Nanopore DNA sequencing can reliably identify fungi in decaying wood. Researchers developed guidelines for quality filtering of Nanopore data to ensure accurate identification of fungal species. They found that specific mycorrhizal fungi, particularly Laccaria amethystina and Tomentella sublilacina, colonize young beech trees growing on decaying logs and help them obtain nutrients.