Research Keyword: PEG-mediated transformation

Establishment of a genetic transformation system for cordycipitoid fungus Cordyceps chanhua

mycolabadmin

Scientists have successfully developed a method to genetically modify Cordyceps chanhua, an important medicinal mushroom used in traditional Chinese medicine for over 1,600 years. By optimizing how they prepare fungal cells and introduce foreign genes, they created a reliable genetic transformation system that can be used to study which genes produce beneficial compounds in this mushroom. This breakthrough will help researchers understand and potentially enhance the medicinal properties of C. chanhua.

Read More »

Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in Cordyceps cicadae

mycolabadmin

Scientists successfully developed a method to genetically modify Cordyceps cicadae, a valuable medicinal fungus used in traditional Chinese medicine. By optimizing how to remove the fungus’s protective cell wall and introducing new genes using a technique called PEG-mediated transformation, researchers created a stable system for genetic manipulation. This breakthrough opens the door for improving medicinal compounds in this fungus and advancing its use in treating various health conditions.

Read More »

Development of Green Fluorescent Protein-Tagged Strains of Fusarium acuminatum via PEG-Mediated Genetic Transformation

mycolabadmin

Scientists developed a method to genetically modify a harmful fungus called Fusarium acuminatum that causes root rot in plants like carnations. Using a technique that breaks down the fungal cell wall and uses a special chemical (PEG) to insert genes, they successfully added a green-glowing protein (GFP) marker to the fungus. This allows researchers to track where and how the fungus infects plants. The modified fungus still behaves normally, making it a useful tool for identifying which genes make the fungus dangerous, potentially leading to better disease control methods.

Read More »

PEG-Mediated Protoplast Transformation of Penicillium sclerotiorum (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

mycolabadmin

Scientists developed a new method to genetically modify a fungus called Penicillium sclerotiorum by using protoplasts, which are fungal cells with their protective outer walls removed. They added a glowing green protein (GFP) to track the fungus and discovered that this modification changed how the fungus uses fats and lipids. When they treated tomato seeds with enzymes before exposing them to the modified fungus, it enhanced the fungus’s ability to colonize plant roots, potentially helping plants grow better.

Read More »

Improved Protoplast Production Protocol for Fungal Transformations Mediated by CRISPR/Cas9 in Botrytis cinerea Non-Sporulating Isolates

mycolabadmin

Scientists have developed a better method to isolate protoplasts (fungal cells without cell walls) from non-sporulating varieties of gray mold fungus. By optimizing the incubation time, culture container, and enzyme used, they produced more viable protoplasts that can regenerate and be genetically modified. This advancement allows researchers to use CRISPR gene-editing technology to understand and potentially control gray mold, which causes significant crop losses worldwide.

Read More »
Scroll to Top