PCR-RFLP – mycolab.ai

Molecular identification of yeast communities isolated from nail specimens by PCR-RFLP and PCR-FSP methods

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This study developed and compared two molecular methods to accurately identify different yeast species that cause nail infections. Researchers tested 269 patient samples and found that both PCR-RFLP and PCR-FSP methods were effective, with PCR-FSP being better at identifying rare yeast species. These methods help doctors identify the exact type of yeast causing infection, which is important for selecting the right antifungal medication for treatment.

A rare case of white piedra caused by Candida orthopsilosis

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A young girl developed small knots on her scalp hair caused by a yeast fungus called Candida orthopsilosis. This is extremely rare, as white piedra is typically caused by different fungi. Doctors identified the fungus using DNA testing and treated it successfully with antifungal creams and shampoo. This case shows that doctors should not dismiss Candida growth as contamination when found on hair.

Molecular Identification of Candida Species among Iranian Patients: Pursuing Candida auris

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Researchers in Iran studied 136 hospital patients to identify different types of Candida fungal infections using a molecular laboratory technique called PCR-RFLP. They found that while Candida albicans remained the most common species, other Candida species were increasingly prevalent in hospitalized patients. The study developed a cost-effective diagnostic method that could help developing countries identify these fungal infections faster and more accurately, though the dangerous multidrug-resistant Candida auris was not found in their samples.

Molecular Identification of Candida Species among Iranian Patients: Pursuing Candida auris

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This study evaluated a simple molecular test called PCR-RFLP to identify different types of Candida fungi in hospitalized patients in Iran. Among 136 patient samples, researchers found eight different Candida species, with common Candida albicans being most prevalent, but other more dangerous species also present. No cases of the emerging pathogen Candida auris were found, though the test method can effectively detect it. The researchers conclude this cost-effective test could be valuable for identifying dangerous fungal infections in developing countries.

Northwest Iranian dermatophyte isolates: anthropophilic and geophilic

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Researchers in northwestern Iran studied fungal infections of skin, hair, and nails by identifying different types of dermatophytes from patient samples and soil. They found that a fungus called Trichophyton mentagrophytes was the most common cause, but also discovered a rare fungus called Myriodontium keratinophilum causing nail infections. Using modern DNA testing methods, they identified seven different dermatophyte species and several other soil fungi, providing important information about fungal infections in this region.

Clinical and Molecular Characterization of Feline Sporotrichosis in the Brazilian Amazon: PCR-Based Identification of Sporothrix brasiliensis

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This research studied a fungal disease called sporotrichosis that affects cats in the Brazilian Amazon and can spread to humans. Most infected cats were young males that roamed freely outdoors and had skin lesions on their faces and paws. Researchers tested different laboratory methods to identify the fungus and found that a specific technique extracted DNA best. They confirmed that Sporothrix brasiliensis was the cause and tested how well common antifungal medications work against it, finding that some drugs work better than others.

Distribution of fungal agents in the respiratory system of patients with underlying lung diseases; molecular identification and antifungal susceptibility profiles

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This research examined fungal infections in hospitalized patients with lung diseases in Iran, identifying which fungi were most common and which antifungal medications worked best against them. Researchers found that Candida albicans and Aspergillus flavus were the most prevalent fungi, particularly in COPD patients. Notably, some Candida isolates showed resistance to certain antifungal drugs, particularly voriconazole. The study emphasizes the importance of proper fungal identification and susceptibility testing for effective treatment.

Addressing filamentous fungi-related onychomycosis in the era of antifungal resistance: assessment of Zataria multiflora nanostructured lipid carrier topical gel in a double-blinded clinical trial

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Nail fungal infections (onychomycosis) are difficult to treat, especially as fungi become resistant to standard antifungal medications. Researchers tested a new gel made from Zataria multiflora (Shirazi thyme) packaged in special nanoparticles that help the medicine penetrate the nail better. In a clinical trial comparing this gel to placebo, the Zataria multiflora treatment showed better results after just 2 weeks, with 70% of patients experiencing fungal cure compared to 55% with placebo.

Research Keyword: PCR-RFLP