Research Keyword: PCR amplification

Detection of Opportunistic Fungi from the Bronchoalveolar Lavage Specimens of Patients with Pulmonary Diseases

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Researchers examined lung fluid samples from patients with lung diseases to identify fungal infections. They found various types of fungi including Aspergillus, Penicillium, and Pneumocystis using microscopy and laboratory tests. Large numbers of fungi were present in many samples, which could interfere with medicine absorption and treatment. The study shows that quick and accurate identification of these fungi is important for treating lung infections properly.

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Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification

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This research improves how scientists extract DNA from fungi found in dried fruits and other sources. The modified CTAB method is faster, cheaper, and produces higher quality DNA than commercial kits, without requiring special equipment like liquid nitrogen. The extracted DNA works well for identifying fungal species and detecting fungal diseases in fish, plants, and humans, making it valuable for both research and medical diagnostics.

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Phylogeny of Aspergillus section Circumdati and inhibition of ochratoxins potential by green synthesised ZnO nanoparticles

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Researchers identified four species of Aspergillus fungus that contaminate crops and produce poisonous compounds called ochratoxins. They found that specially made zinc oxide nanoparticles, created using an environmentally friendly method with basil extract, can significantly reduce the amount of ochratoxins produced by these fungi. This discovery could help protect agricultural products from contamination and improve food safety.

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Geographical distribution of Cryptococcus neoformans in Iran, pathogenic factors, and antifungal susceptibility profile

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Researchers tested pigeon droppings from across Iran to find a dangerous fungus called Cryptococcus neoformans that can cause serious lung infections. They found this fungus in about 8.6% of samples, with more present in some provinces than others. The fungus showed concerning resistance to some common antifungal medications but remained sensitive to others like voriconazole, which could help guide treatment decisions.

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Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification

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Researchers developed an improved method for extracting fungal DNA that is faster, cheaper, and safer than current commercial kits. The new protocol works by using a special chemical solution and simple crushing tools instead of expensive equipment like liquid nitrogen or bead beaters. They tested it on 40 different fungal species found in dry fruits and successfully identified them all using genetic markers, showing the method could help diagnose fungal diseases in plants, animals, and humans.

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