next-generation sequencing – Page 2

Genome Sequencing of Hericium coralloides by a Combination of PacBio RS II and Next-Generation Sequencing Platforms

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Scientists successfully sequenced the complete genome of Hericium coralloides, a medicinal mushroom used in traditional Chinese medicine for treating heart disease, cancer, and stomach problems. Using advanced sequencing technology combining long-read and short-read methods, they assembled the 55 million base pair genome containing over 11,700 genes. This genetic blueprint reveals the mushroom contains genes for producing beneficial enzymes and compounds with therapeutic potential. This milestone opens the door for future research to develop new medicines based on this mushroom’s natural bioactive properties.

Changes in intra-host mycovirus population diversity after vertical and horizontal transmission

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Researchers studied how a virus that helps control chestnut blight disease changes when it spreads between fungal cells. They found that when the virus passes through spores, it becomes less diverse, but when it spreads between fungal cells, diversity is maintained better. The fungal host’s genetics strongly influences how many viral variants survive transmission, which has important implications for using this virus as a disease control strategy.

Clinical aspects and recent advances in fungal diseases impacting human health

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Fungal infections are becoming a major health threat, affecting over a billion people worldwide. The main problems are difficulty diagnosing these infections, increasing resistance to current medications, and limited treatment options. Doctors and the public need better awareness, and new antifungal drugs with different approaches are needed to effectively treat resistant infections.

Complete genome sequence of Diaporthe vaccinii Shear, a fungal isolated from blueberry

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Scientists sequenced the complete genetic blueprint of Diaporthe vaccinii, a harmful fungus that damages blueberry plants and costs farmers significant money. Using advanced DNA sequencing technology, they assembled a high-quality genetic map of the fungus to better understand how it causes disease. This genetic information could help researchers develop better ways to protect blueberry crops from this destructive pathogen.

The first described case of Fomitiporella micropora infection in humans: A heart transplant recipient diagnosed by fungal PCR

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A patient who received a heart transplant developed a small, slowly-growing lump on his shin over many years. After surgical removal, genetic testing revealed it was caused by a rare fungus never before identified in humans. The surgical removal likely cured the infection, and the case shows how important advanced genetic testing methods are for identifying unusual infections in transplant patients.

Acute Respiratory Distress Syndrome Due to COVID-19-Associated Pulmonary Aspergillosis with Rare Extensive Tracheobronchial Pseudomembranous Involvement: A Case Report

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An 87-year-old man with COVID-19 developed a rare and severe fungal infection in his airways caused by Aspergillus fumigatus. Doctors used a camera tube (bronchoscope) to see thick, yellowish-white membrane-like material coating his entire windpipe and airways, which is an extremely rare complication. Despite starting antifungal medications, his condition rapidly worsened leading to organ failure and death. This case highlights how COVID-19 can leave patients vulnerable to serious secondary fungal infections that require immediate recognition and treatment.

Analytical Validation of a Next-Generation Sequencing Assay for the Detection of Microbial Pathogens in Human Plasma

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Researchers developed a new blood test that can detect hundreds of different fungal and bacterial infections using advanced sequencing technology. The test works by searching for pathogen DNA in blood samples and is much faster than traditional culture methods. This could help doctors quickly identify what infection a patient has and start the right treatment sooner, which is especially important for patients with weakened immune systems.

Acute Respiratory Distress Syndrome Due to COVID-19-Associated Pulmonary Aspergillosis with Rare Extensive Tracheobronchial Pseudomembranous Involvement: A Case Report

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An elderly COVID-19 patient developed a rare and severe fungal infection caused by Aspergillus fungus in the airways, creating yellowish-white membrane-like deposits that blocked breathing. Despite identification of the fungus through examination and modern genetic testing, and treatment with antifungal medications, the patient’s condition worsened rapidly leading to organ failure and death. This case highlights how fungal infections can complicate COVID-19, especially in elderly patients on multiple medications, and stresses the importance of early recognition and immediate antifungal treatment.

Microbial decay of wooden structures: actors, activities and means of protection

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Wood is an increasingly popular sustainable building material, but fungi and bacteria can cause significant damage to wooden structures. This review explains how different types of fungi and bacteria decay wood through various mechanisms, describes advanced DNA methods to identify these microorganisms, and outlines practical strategies to protect wood. By combining proper design, chemical treatments, and wood modification techniques, builders can extend the lifespan of wooden structures while maintaining environmental sustainability.

De Novo Genome Assembly and Comparative Genome Analysis of the Novel Human Fungal Pathogen Trichosporon austroamericanum Type-Strain CBS 17435

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Scientists sequenced the complete genome of a dangerous fungal species called Trichosporon austroamericanum that can cause serious infections in humans, particularly transplant patients. Using advanced long-read sequencing technology, they assembled the organism’s 21 million base pair genome and compared it to a closely related fungal species. The analysis showed this species is genetically distinct and has interesting characteristics that help it survive at higher temperatures than most other fungi. This genetic information will help doctors and researchers better understand and treat infections caused by this emerging pathogenic yeast.

Research Keyword: next-generation sequencing