Research Keyword: mycotoxin detection

Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

This study developed a fast molecular test using real-time PCR to detect harmful Aspergillus fungi in grains and seeds. Instead of waiting 3-5 days for traditional culture methods, this new test can identify the fungi in just hours by detecting specific DNA patterns. The test was tested on 140 samples of cereals and oilseeds and proved more accurate than traditional methods, making it useful for food safety in markets and processing plants.

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From mold to mycotoxins: an LC–MS/MS method for quantifying airborne mycotoxins in indoor environments

Mold in water-damaged buildings produces toxic compounds called mycotoxins that can become airborne and be breathed in. This study developed a precise laboratory method to detect and measure 29 different mycotoxins in indoor air samples. Researchers tested the method in three real mold-infested buildings and successfully identified seven different mycotoxins, proving the method works well even when toxin levels are very low. This breakthrough helps determine whether moldy indoor environments pose serious health risks to workers and occupants.

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Real-time CO2 monitoring for early detection of grain spoilage and mycotoxin contamination

This research shows that monitoring carbon dioxide levels in stored grain is a better way to detect dangerous mold contamination than measuring temperature alone. Scientists tested this approach in both small laboratory containers and larger industrial grain silos over nine months, finding that CO2 levels rise rapidly when grain gets wet and fungal contamination begins. This early warning system could help food producers quickly take action to prevent spoilage and contamination with harmful toxins that can make people sick.

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Design of a melting curve analysis (MCA) based on multiplex real-time PCR for detection of Aspergillus terreus and Aspergillus fumigatus in cereals and oilseeds samples

This study developed a fast molecular test to detect harmful fungi (Aspergillus species) in grains and seeds. Traditional fungal identification takes 3-5 days, but the new PCR-based method can produce results in hours. Testing 140 grain and seed samples, the new method successfully identified two dangerous Aspergillus species that produce toxic compounds harmful to human health. This advancement helps ensure food safety by enabling quicker detection of contamination in food production facilities.

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