molecular markers – Page 2

Editorial: Unraveling pathogen-plant-microbiome interactions in horticultural crops through omics approaches

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This editorial presents a collection of research on how diseases affect valuable crops like tomatoes, tea, and potatoes. Scientists used advanced genetic and molecular techniques to understand how pathogens infect plants and how the beneficial microbes around plant roots can help fight disease. The studies suggest that managing crop diseases sustainably requires understanding the complex interactions between pathogens, plants, and their microbial communities.

Novel and advanced MNP molecular markers accurately identify the genetic similarity of Hypsizygus marmoreus strains: a comparative evaluation with ISSR and antagonistic methods

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Researchers developed a new genetic fingerprinting method using 369 molecular markers to accurately identify different strains of H. marmoreus mushrooms. By analyzing the DNA sequences of 32 strains, they found that no two strains were genetically identical, with significant diversity patterns observed between white and gray varieties. This new method proved more reliable and efficient than traditional testing approaches, helping prevent the problem of identical mushroom strains being sold under different names.

New Species of Ascomycetes from Two Hypersaline Endorheic Lagoon Complexes in Zaragoza Province (Aragon Community, Spain)

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Scientists discovered three new types of fungi living in extremely salty lagoons in Spain. These fungi can survive in harsh conditions that kill most organisms. The researchers identified these new species by studying their shape and DNA, and also reorganized how some existing fungi are classified based on genetic relationships. This discovery helps us understand how life adapts to extreme environments.

A New Method for Constructing High-Resolution Phylogenomic Topologies Using Core Gene-Associated MNP Markers: A Case Study From Agaricus bisporus

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Scientists developed a new method to accurately identify and distinguish different strains of button mushrooms using genetic markers derived from core genes. This method is more accurate and simpler than previous approaches and can be applied to other mushroom species and fungi. The research helps mushroom farmers and breeders properly identify cultivars and protect their varieties from unauthorized propagation.

Endophytic Fungi Isolated from the Marine Macroalga Dictyopteris pacifica in Korea

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Researchers in Korea discovered three species of fungi living inside a brown seaweed called Dictyopteris pacifica. These fungi were previously unknown in Korea and were identified using genetic analysis. The fungi have mutualistic relationships with the seaweed and may produce compounds useful for medicines and treating diseases.

Three new species of Talaromyces sect. Talaromyces discovered in China

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Scientists discovered three new species of Talaromyces fungi in Chinese soil samples. These fungi are important because they can break down plant material, produce useful enzymes for industries, and create compounds with medical potential. However, some species in this group can cause infections in people with weakened immune systems. The new species were identified by examining their physical characteristics and comparing their genetic sequences.

New insights into Cortinarius: Novel taxa from subtropical China

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Scientists discovered six new types of Cortinarius mushrooms in subtropical forests of China. These mushrooms form symbiotic relationships with tree roots. The researchers used genetic analysis combined with physical characteristics to identify and describe these new species, adding significantly to our understanding of fungal diversity in this region.

On site discrimination between two closely related commercial strains of oyster mushroom using a loop-mediated isothermal amplification (LAMP) test

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Scientists developed a quick test to identify two specific types of oyster mushrooms (SPOPPO and ALLERPO) on farms or spawn production facilities. These sporeless mushroom varieties were created to protect workers from respiratory problems caused by mushroom spores. The new LAMP test can identify which strain is being grown in just 30 minutes using simple sample preparation, helping protect the breeding companies’ intellectual property rights from illegal copying.

High-Resolution Core Gene-Associated Multiple Nucleotide Polymorphism (cgMNP) Markers for Strain Identification in the Wine Cap Mushroom Stropharia rugosoannulata

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Scientists developed a new genetic tool called cgMNP markers to accurately identify different strains of wine cap mushrooms (Stropharia rugosoannulata). By analyzing the DNA of 105 mushroom strains collected from across China, they discovered that a single gene containing 865 genetic variations was sufficient to distinguish between all cultivated varieties. This breakthrough provides a practical way for mushroom farmers and researchers to identify specific strains quickly, which is important for breeding better mushrooms and maintaining quality in commercial production.

Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees

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Scientists developed an optimized method for growing brown spot needle blight fungi from infected pine needles and extracting their DNA for research. They tested four different growth media and four DNA extraction techniques to find the best combination. Sabouraud dextrose medium combined with a CTAB-based extraction method containing polyvinylpyrrolidone worked best, producing high-quality DNA suitable for advanced genetic studies. This standardized approach will help researchers better understand this important forest disease.

Research Keyword: molecular markers