molecular markers – mycolab.ai

Molecular Markers for Detecting a Wide Range of Trichoderma spp. that Might Potentially Cause Green Mold in Pleurotus eryngii

mycolabadmin

King oyster mushrooms are susceptible to green mold caused by various Trichoderma fungi, which significantly reduces crop yields. Researchers developed a quick DNA test that can detect these harmful fungi in mushroom farms before they cause major damage. The test is sensitive enough to identify the fungus even when present in very small amounts mixed with mushroom tissue. This new detection tool will help mushroom farmers monitor their crops and prevent costly contamination losses.

Characterization of Homeodomain Proteins at the Aβ Sublocus in Schizophyllum commune and Their Role in Sexual Compatibility and Development

mycolabadmin

This study reveals how a split-gill mushroom called Schizophyllum commune controls its sexual reproduction and fruiting body development through specific protein interactions. Scientists identified four key proteins at a genetic locus that work together in pairs to enable sexual compatibility between different mushroom strains. Understanding these genetic mechanisms helps create improved varieties of this edible and medicinal mushroom with better nutritional and pharmaceutical properties.

Genome-Wide SSR Markers Reveal Genetic Diversity and Establish a Core Collection for Commercial Hypsizygus marmoreus Germplasm

mycolabadmin

Researchers developed a streamlined genetic database for beech mushrooms (H. marmoreus) by analyzing 57 strains and identifying genetic markers. They selected 24 representative strains that capture all the genetic diversity of the larger collection, making breeding programs more efficient. Each strain received a unique molecular ID code similar to a genetic barcode for easy identification and authentication in commercial breeding and cultivation.

Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in Cordyceps cicadae

mycolabadmin

Scientists successfully developed a method to genetically modify Cordyceps cicadae, a valuable medicinal fungus used in traditional Chinese medicine. By optimizing how to remove the fungus’s protective cell wall and introducing new genes using a technique called PEG-mediated transformation, researchers created a stable system for genetic manipulation. This breakthrough opens the door for improving medicinal compounds in this fungus and advancing its use in treating various health conditions.

Holarctic Species in the Pluteus podospileus Clade: Description of Six New Species and Reassessment of Old Names

mycolabadmin

Scientists have discovered and formally identified six new species of mushrooms in the Pluteus podospileus group that was previously thought to be a single variable species across the Northern Hemisphere. Using DNA analysis combined with traditional microscopic examination, researchers found that what was once considered one widespread species is actually multiple distinct species with different geographic distributions in Europe, Asia, and North America. The study also resurrected an old, forgotten species name and clarified the relationships between various populations of these small brown mushrooms that grow on decaying wood in forests.

New Species of Ascomycetes from Two Hypersaline Endorheic Lagoon Complexes in Zaragoza Province (Aragon Community, Spain)

mycolabadmin

Scientists discovered three new species of fungi living in extremely salty lagoons in Spain. These fungi were identified using both traditional microscopy and DNA analysis. The research also reorganized how some existing fungi are classified scientifically. This study helps us better understand the hidden microbial life in salt lakes around the world.

Morphological and molecular development of Terfezia claveryi ectendomycorrhizae exhibits three well-defined stages

mycolabadmin

This study tracked how desert truffles form a beneficial partnership with plant roots over 10 weeks, identifying three distinct stages with different structures and genetic activity patterns. Researchers used microscopy and gene analysis to understand how the fungus gradually colonizes the root system, starting with growth in soil, then spreading between root cells, and finally penetrating inside cells. The findings reveal that specific fungal and plant genes are active at different stages, particularly those involved in breaking down plant cell walls. This research helps explain how desert truffles can be cultivated more effectively for food production.

Development of Green Fluorescent Protein-Tagged Strains of Fusarium acuminatum via PEG-Mediated Genetic Transformation

mycolabadmin

Scientists developed a method to genetically modify a harmful fungus called Fusarium acuminatum that causes root rot in plants like carnations. Using a technique that breaks down the fungal cell wall and uses a special chemical (PEG) to insert genes, they successfully added a green-glowing protein (GFP) marker to the fungus. This allows researchers to track where and how the fungus infects plants. The modified fungus still behaves normally, making it a useful tool for identifying which genes make the fungus dangerous, potentially leading to better disease control methods.

Ectophoma salviniae sp. nov., Neottiosporina mihintaleensis sp. nov. and four other endophytes associated with aquatic plants from Sri Lanka and their extracellular enzymatic potential

mycolabadmin

Researchers in Sri Lanka discovered six species of fungi living inside freshwater aquatic plants, including two entirely new species. These fungi were identified using advanced genetic testing and were found to produce useful enzymes like amylase, cellulase, and laccase. This research reveals that Sri Lankan freshwater ecosystems harbor diverse fungal communities with potential biotechnological applications.

Barcode high-resolution melting (Bar-HRM) analysis to authenticate true cinnamon (Cinnamomum verum) from its adulterants and contaminants

mycolabadmin

This study developed a rapid and cost-effective DNA test to verify that cinnamon products are authentic true cinnamon (Ceylon cinnamon) rather than cheaper substitutes. The test uses DNA barcoding and melting curve analysis to distinguish true cinnamon from three common adulterants and can also detect contamination with a toxic fungus. The method is particularly useful for processed cinnamon products like powder where traditional identification methods don’t work, helping protect consumers and maintain market integrity for authentic Sri Lankan cinnamon.

Research Keyword: molecular markers