Research Keyword: molecular biology

Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi

Scientists developed a practical method to extract high-quality DNA from mushrooms collected in the wild, even in remote locations without refrigeration. The technique involves preserving fresh samples in alcohol and then carefully purifying the DNA through multiple steps. This method successfully produced DNA suitable for reading the complete genetic code of 23 different fungal species, particularly those that cannot be grown in laboratories.

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Chromosome-Wide Characterization of Intragenic Crossover in Shiitake Mushroom, Lentinula edodes

This study examines how genetic material is shuffled during reproduction in shiitake mushrooms. Scientists identified 65 regions in the mushroom genome where genetic crossovers happen more frequently. They discovered that special DNA sequences and specific genes regulate where these crossovers occur. Understanding these patterns can help improve mushroom breeding programs by making it easier to combine desirable traits.

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