Research Keyword: microscopy

Assessing the Validity and Impact of Remote Digital Image Reading in Fungal Diagnostics

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This study tested whether trained mycologists could accurately identify fungal infections from digital images viewed remotely, similar to how radiologists review X-rays. Five experienced laboratory professionals analyzed 474 images of different fungi with accuracy rates between 78-93%. The results suggest that remote digital diagnosis could help hospitals in developing countries where expert mycologists are scarce, enabling faster and more accurate diagnosis of serious fungal infections.

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Intracellular accommodation of bacteria, fungi, and oomycetes by plants analyzed using transmission electron microscopy

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Scientists used high-powered electron microscopes to examine how plants host different microorganisms inside their cells. They found that whether the microorganism is a helpful nitrogen-fixing bacterium, a nutrient-exchanging fungus, or a disease-causing oomycete, plants always separate it from the rest of the cell with a special membrane. This study reveals fundamental similarities in how plants accommodate different types of microorganisms, despite the very different outcomes for the plant.

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Not everything that wiggles is a worm: Pseudoparasites in parasitology

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When examining patient samples under a microscope, parasitologists must be careful to distinguish real parasites from artifacts that only look like parasites. Items such as pollen, plant fibers, yeast, and food remnants can closely resemble parasitic organisms and lead to incorrect diagnoses and unnecessary treatment. By using proper training, multiple diagnostic techniques, and careful morphological evaluation, healthcare professionals can avoid these diagnostic errors and ensure accurate patient care.

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Accelerated protein retention expansion microscopy using microwave radiation

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Scientists have developed a faster way to examine brain tissue at extremely small scales using a combination of expansion microscopy and microwave radiation. Instead of waiting days for tissue samples to process, the new method cuts the time down to hours while maintaining the same quality of detailed images. The technique was successfully tested on tadpole and fruit fly brains, showing it could be useful for studying neural structures and potentially applied to studying brain diseases like Alzheimer’s.

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Scanning electron microscopy of hyphal ectobiont bacteria within mycelial extracellular matrices

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Researchers studied how bacteria attach to fungal filaments using advanced microscopy. They developed a special preparation method that removed outer biofilm layers to reveal how bacteria stick to fungal structures. The study found that bacteria form attachment structures with the fungal surface, with these structures being primarily produced by the fungus. This research helps us understand how bacteria and fungi interact in nature and in biotechnology.

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Prevalence of Tinea pedis in military personnel: a systematic review and meta-analysis

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Athlete’s foot is a common fungal infection affecting military personnel at rates around 17% globally. Military environments create perfect conditions for this infection through close living quarters, humidity from sweat and boots, and shared facilities. The study found that regions with hot, humid climates have higher infection rates, and proper diagnosis using both microscopy and culture tests is important for accurate detection. Improved hygiene, better-designed footwear, and access to antifungal treatments can significantly reduce infection rates in military settings.

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