Research Keyword: loop-mediated isothermal amplification

Rapid and Sensitive Detection of Verticillium dahliae from Soil Using LAMP-CRISPR/Cas12a Technology

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Scientists developed a new rapid test to detect a dangerous fungal disease that affects cotton crops. The test combines two advanced molecular techniques (LAMP and CRISPR) to quickly identify the disease-causing fungus in soil samples. The system works in laboratories but can also be used in fields by farmers, taking less than 2 hours to produce results. This breakthrough will help farmers catch and manage the disease early, protecting their crops and improving yields.

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Aspergillus in Italian Pistachios: Characterization and Detection of Major Aflatoxigenic Species With a Loop-Mediated Isothermal Amplification Assay

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Researchers studied pistachio nuts from Italy and found dangerous mold species that produce aflatoxins, harmful toxins that can cause serious health problems. They developed a quick and reliable test using molecular biology techniques to detect these molds in pistachios, even at very low levels of contamination. The test uses specially treated dried primers that remain stable for over a month, making it practical for food safety monitoring in warehouses and markets around the world.

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On site discrimination between two closely related commercial strains of oyster mushroom using a loop-mediated isothermal amplification (LAMP) test

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Scientists developed a quick test to identify two specific types of oyster mushrooms (SPOPPO and ALLERPO) on farms or spawn production facilities. These sporeless mushroom varieties were created to protect workers from respiratory problems caused by mushroom spores. The new LAMP test can identify which strain is being grown in just 30 minutes using simple sample preparation, helping protect the breeding companies’ intellectual property rights from illegal copying.

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Simultaneous Detection of Four Madurella Species Using Loop-Mediated Isothermal Amplification (LAMP) for Eumycetoma Diagnosis

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Scientists developed a simple new test to identify four types of fungi that cause a serious tropical disease called eumycetoma. Unlike previous tests that required expensive equipment and multiple reactions, this new test works in one tube using simple heating instead of complex thermal cycling. The test can detect the fungi in about 45 minutes and works reliably in remote areas with limited resources.

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