long-read sequencing

Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi

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Scientists developed a practical method to extract high-quality DNA from mushrooms collected in the wild, even in remote locations without refrigeration. The technique involves preserving fresh samples in alcohol and then carefully purifying the DNA through multiple steps. This method successfully produced DNA suitable for reading the complete genetic code of 23 different fungal species, particularly those that cannot be grown in laboratories.

Sanctuary: a Starship transposon facilitating the movement of the virulence factor ToxA in fungal wheat pathogens

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Researchers found that a disease-causing gene called ToxA, which helps fungal pathogens infect wheat crops, travels between different fungal species using molecular ‘cargo ships’ called Starship transposons. By sequencing multiple fungal isolates, scientists discovered that ToxA rides within a larger mobile genetic element called Sanctuary that can move around within fungal genomes and between species. This discovery helps explain how wheat pathogens become more dangerous over time through horizontal gene transfer.

Morphogenesis, starvation, and light responses in a mushroom-forming fungus revealed by long-read sequencing and extensive expression profiling

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Researchers created a detailed genetic instruction manual for a common mushroom species called Coprinopsis cinerea. Using advanced sequencing technology, they identified all the genes and precisely mapped where genes start and stop, what controls them, and how they respond to light and hunger. This improved genetic map reveals how mushrooms form fruiting bodies and survive changing environmental conditions, providing a valuable resource for understanding mushroom biology and improving mushroom cultivation.

Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand

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Researchers in Thailand studied a fungal infection called sporotrichosis by using advanced DNA sequencing technology to identify the exact species and understand how different strains are related to each other. They found that while current treatment options work, some strains are becoming resistant to the main drug used (itraconazole). The study shows that a newer, faster DNA sequencing method can be just as reliable as traditional methods for identifying these dangerous fungi and tracking how they spread between cats and humans.

Genome resource of Phlyctema vagabunda strain 19EL15, a pathogen of post-harvest bull’s eye rot of apple

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Scientists sequenced the complete genetic blueprint of a fungus called Phlyctema vagabunda that causes serious damage to apples and pears after harvest, particularly creating brown spots called bull’s eye rot. The fungus is found across Europe and North America and costs farmers significant money in crop losses. This genetic information will help researchers better understand how the fungus works and develop better ways to prevent or manage the disease.

Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand

mycolabadmin

This research examined a fungal infection called sporotrichosis found in Thailand, particularly in cats and humans. Scientists used advanced DNA sequencing technology to accurately identify which species of the fungus caused the infections and understand how they are related to each other. They also tested how well common antifungal medications work against these fungi, finding that some strains have become resistant to itraconazole, the main drug used to treat sporotrichosis.

Improved Extraction Methods to Isolate High Molecular Weight DNA From Magnaporthaceae and Other Grass Root Fungi for Long-Read Whole Genome Sequencing

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Scientists have developed improved methods to extract high-quality DNA from pathogenic fungi, which is essential for sequencing and understanding how these fungi cause plant diseases. Two different extraction techniques were refined and tested on Magnaporthaceae fungi: one that works with small amounts of fungal material, and another that produces longer DNA strands when more material is available. The key to success is harvesting the fungi before they accumulate dark pigments that interfere with DNA extraction. These protocols will help researchers create better genome maps of disease-causing fungi, leading to improved strategies for crop protection.

Methodology for Extracting High-Molecular-Weight DNA from Field Collections of Macrofungi

mycolabadmin

Scientists developed a practical method to extract high-quality DNA from mushrooms found in nature, which is essential for understanding fungal genomes. The technique works without needing freezers or ice by preserving tissue in alcohol at room temperature. The method successfully extracted usable DNA from 33 different mushroom species, including rare species that cannot be grown in laboratories, enabling researchers to sequence and study their complete genomes.

Research Keyword: long-read sequencing