Research Keyword: limit of detection

Development of reverse transcription loop-mediated isothermal amplification-based assay for rapid and specific detection of human fungal pathogen, Candida auris

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Researchers developed a new rapid test called RT-LAMP to detect Candida auris, a dangerous multidrug-resistant fungus that causes hospital infections. The test works by changing color from pink to yellow when the fungus is present, making it easy to read without special equipment. It is more sensitive and specific than current methods, can be done quickly at a single temperature, and costs much less than existing tests, making it ideal for hospitals and clinics with limited resources.

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An Optimized In-House Protocol for Cryptococcus neoformans DNA Extraction from Whole Blood: Comparison of Lysis Buffer and Ox-Bile Methods

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Researchers developed a simple, low-cost method to extract DNA from Cryptococcus neoformans fungal cells found in blood samples. The new method uses a homemade salt buffer solution instead of expensive commercial kits, making it accessible to laboratories in developing countries. Testing showed this method works better than an alternative ox-bile method, detecting very small numbers of fungal cells. This breakthrough could help doctors more quickly diagnose a serious fungal infection called cryptococcal meningitis in resource-limited settings.

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Performance Evaluation of Five Real-Time PCR Assays for the Detection of Candida auris DNA

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Candida auris is a dangerous fungal infection that spreads in hospitals and resists common antifungal medications. Researchers compared five different rapid tests (PCR assays) to see which ones best detect this pathogen. The study found that different tests vary widely in their ability to detect the fungus, with some being much more sensitive than others. The findings highlight the need for standardized testing methods worldwide to ensure hospitals can reliably identify and control C. auris infections.

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Multicenter performance evaluation of the “quanty TOXO (RH region)” kit (Clonit) for molecular diagnosis of toxoplasmosis

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Researchers from seven French hospitals tested a commercial DNA test kit called “quanty TOXO” that detects Toxoplasma gondii, a parasite causing serious infections in pregnant women and people with weakened immune systems. The kit performed well overall, correctly identifying infection in nearly 95% of positive cases and showing no false positives. However, the test sometimes missed infections when parasite levels were very low, suggesting doctors should run tests twice to increase detection chances.

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