ITS sequencing – Page 9

Investigating fungal diversity through metabarcoding for environmental samples: assessment of ITS1 and ITS2 Illumina sequencing using multiple defined mock communities with different classification methods and reference databases

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Scientists developed a comprehensive method for identifying different fungi in environmental samples using DNA sequencing technology called metabarcoding. They tested 37 mixtures of known fungi species to compare different approaches, including which DNA markers to use, which reference databases to search, and which computer analysis methods to apply. The study found that the choice of method significantly affects results, with some approaches better at genus-level identification and others at species identification, helping researchers select the best approach for their specific needs.

Root zone microbial communities of Artemisia ordosica Krasch. at different successional stages in Mu US Sandy Land: a metagenomic perspective with culturomics insights

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Researchers studied the bacteria and fungi living around the roots of a desert plant called Artemisia ordosica that helps prevent sand dunes from spreading in China. By analyzing DNA and growing microbes in the lab, they found different communities of microorganisms at different stages of sand dune recovery. Key beneficial microbes like Bacillus and Penicillium were identified, which may help the plant survive in harsh, nutrient-poor sandy soils.

Cadmium and Lead Tolerance of Filamentous Fungi Isolated from Contaminated Mining Soils

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Researchers found several types of fungi thriving in extremely contaminated mining soils in Mexico. These fungi can survive in environments with toxic levels of lead and cadmium that would kill most organisms. The most promising fungus, Paecilomyces lilacinus, can tolerate both metals and could potentially be used to clean up heavily polluted mining sites by binding and immobilizing these dangerous metals in the soil.

Basidiobolomycosis caused by a rare species – Basidiobolus meristosporus

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A five-year-old girl developed large swollen lumps on her buttock and thigh that were initially thought to be tuberculosis or cancer. Doctors took a tissue sample and found it was caused by a rare fungus called Basidiobolus meristosporus. Using advanced DNA sequencing techniques, they identified the exact fungus species and treated her successfully with an antifungal medication called itraconazole, leading to complete healing within six weeks.

The association of Yarrowia lipolytica with onychomycosis

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A 20-year-old woman suffered from a persistent toenail infection that didn’t respond to several standard antifungal treatments over five years. Researchers identified the culprit as Yarrowia lipolytica, a rare yeast not previously known to cause nail infections. Testing showed this yeast was resistant to common antifungal medications the patient had received, explaining why previous treatments failed. This unusual case highlights the importance of properly identifying fungal pathogens and testing them for drug resistance before starting treatment.

Spectrum of Fungal Infections in Continuous Ambulatory Peritoneal Dialysis: A 20-Year Retrospective Study From a Tertiary Care Center

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This study examined fungal infections in patients undergoing continuous ambulatory peritoneal dialysis (CAPD) over 20 years at a major Indian hospital. Researchers identified various fungal species causing peritonitis, with Candida species being most common, but discovered increasingly rare fungi involved in these infections. The study found that proper identification of the specific fungus and testing for drug resistance is crucial for effective treatment, typically involving catheter removal and targeted antifungal medications.

Isolation and characterization of a new Leptobacillium species promoting tomato plant growth

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Researchers discovered a new beneficial fungus called Leptobacillium that lives inside tomato plant roots without causing harm. When tomato seeds were treated with this fungus, the plants grew better, had more chlorophyll in their leaves, and produced tastier fruits with higher levels of lycopene, a beneficial compound in tomatoes. This discovery suggests the fungus could be used to improve tomato crop production naturally, reducing the need for chemical inputs and helping plants cope with heat stress.

Morphological, Physiological, Biochemical, and Molecular Characterization of Fungal Species Associated with Papaya Rot in Cameroon

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Papaya rot is a major problem for farmers in Cameroon, causing significant losses in this important tropical fruit crop. Researchers identified three fungi responsible for this rot: Colletotrichum gloeosporioides, Fusarium equiseti, and Lasiodiplodia theobromae. These fungi produce enzymes that break down papaya tissue and also produce toxins harmful to consumers. Understanding how these fungi grow and what conditions favor their development can help farmers develop better strategies to prevent rot and reduce post-harvest losses.

Fungal Assemblages in Northern Elms—Impacts of Host Identity and Health, Growth Environment, and Presence of Dutch Elm Disease

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This study examined the fungi living in elm tree shoots to understand how different elm species, tree health, and location affect fungal communities. Researchers found that diseased elms had more diverse fungi than healthy ones, and that a fungus called Sphaeropsis ulmicola may be as damaging as the classic Dutch elm disease pathogen. Urban elm trees hosted more different types of fungi than rural ones, suggesting that city conditions influence which fungi can grow on elms.

Black mold in the bag room: First case of Arthrocladium tropicale peritonitis in a peritoneal dialysis patient

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An 82-year-old dialysis patient developed an unusual infection caused by a black mold called Arthrocladium tropicale, which had never been documented in humans before. The infection was traced to poor storage conditions and ant infestations near the patient’s dialysis supplies in Thailand during rainy season. Doctors identified the rare fungus using molecular genetic testing and treated it successfully with antifungal medication combined with removal of the dialysis catheter. This case highlights the importance of keeping dialysis equipment in clean, dry environments and preventing pest access to prevent serious infections.

Research Keyword: ITS sequencing