ITS region – Page 2

Four Unrecorded Species of Endophytic Diaporthe (Sordariomycetes) in Korea

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Researchers in Korea discovered four previously unknown species of Diaporthe fungi living inside plant tissues without causing visible damage. These fungi were identified using genetic analysis and compared to known species from around the world. The findings show that Korea has more fungal diversity than previously documented and suggest these fungi could potentially be used to develop new medicines or help control plant diseases.

What Quality Suffices for Nanopore Metabarcoding? Reconsidering Methodology and Ectomycorrhizae in Decaying Fagus sylvatica Bark as Case Study

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This study shows that Nanopore DNA sequencing can reliably identify fungi in decaying wood. Researchers developed guidelines for quality filtering of Nanopore data to ensure accurate identification of fungal species. They found that specific mycorrhizal fungi, particularly Laccaria amethystina and Tomentella sublilacina, colonize young beech trees growing on decaying logs and help them obtain nutrients.

Targeted long-read sequencing analysis and antifungal susceptibility profiles of Sporothrix schenckii isolates from Thailand

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This research examined a fungal infection called sporotrichosis found in Thailand, particularly in cats and humans. Scientists used advanced DNA sequencing technology to accurately identify which species of the fungus caused the infections and understand how they are related to each other. They also tested how well common antifungal medications work against these fungi, finding that some strains have become resistant to itraconazole, the main drug used to treat sporotrichosis.

Ascoidea xinghuacunensis sp. nov., a novel ascomycetous yeast species from Xinghuacun Fenjiu old workshop, Shanxi province of China

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Scientists discovered a new type of heat-loving yeast in a traditional Chinese liquor-making workshop in Shanxi province. This yeast, named Ascoidea xinghuacunensis, is the first member of its rare genus to be found in China and can survive at higher temperatures than its relatives. The discovery was made by analyzing the genetic material and physical characteristics of yeast samples collected from fermentation facilities, expanding our knowledge of microscopic life in food production environments.

The Expanding Truffle Environment: A Study of the Microbial Dynamics in the Old Productive Site and the New Tuber magnatum Picco Habitat

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Researchers studied how microbial communities (fungi and bacteria) develop in expanding white truffle forests compared to established productive forests in Tuscany, Italy. Over two years, they found that new forest areas have more diverse and unstable microbial communities with rapid colonization by various fungi, while older forests show more stable communities dominated by truffle-associated fungi. The study reveals how forest management practices, like selective cutting, influence the balance between fungi that break down plant material and those that form beneficial relationships with tree roots, suggesting that understanding these microbial dynamics is key to successfully expanding truffle cultivation.

The Expanding Truffle Environment: A Study of the Microbial Dynamics in the Old Productive Site and the New Tuber magnatum Picco Habitat

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This study examined the microbial communities in white truffle forests in Italy, comparing an established productive forest with a nearby expanding area. Researchers found that the expanding area had more diverse fungal communities with opportunistic species like Mortierella, while the mature forest had a more stable community dominated by ectomycorrhizal fungi. The study identified specific bacteria like Sphingomonas that showed positive associations with white truffles, suggesting these microbes may play important roles in truffle development and could help guide future cultivation efforts.

Four Unrecorded Species of Endophytic Diaporthe (Sordariomycetes) in Korea

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Researchers in Korea discovered four new species of endophytic fungi called Diaporthe living inside plant tissues without causing visible disease. Using genetic testing and microscopic examination, they identified D. caryae, D. phoenicicola, D. stewartii, and D. unshiuensis as previously unrecorded in Korea. These fungi can potentially produce compounds with medicinal properties or help manage plant diseases. This discovery helps scientists better understand the hidden fungal diversity in Korean ecosystems.

Protein Coding Low-Copy rpb2 and ef1-α Regions Are Viable Fungal Metabarcoding DNA Markers Which Can Supplement ITS for Better Accuracy

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Researchers tested different DNA markers for identifying fungal species using DNA sequencing technology. They compared the standard fungal marker (ITS) with two alternative protein-coding markers (rpb2 and ef1-α) on closely related mushroom species. The results showed that using multiple markers together provides better and more reliable identification of fungal species compared to using just one marker, which is especially useful for environmental monitoring and species identification studies.

Torulaspora lindneri sp. nov., a novel ascomycetous yeast species isolated from China and Thailand

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Scientists discovered and formally described a new yeast species called Torulaspora lindneri, found in chestnut tree bark in China and soil in Thailand. This yeast belongs to a group known for various applications in fermentation and biotechnology. The species was identified through genetic sequencing and microscopic examination, showing it forms spherical spores with distinctive tapered structures. The findings contribute to our understanding of yeast diversity in natural forest environments.

Elucidation of Twig Canker and Shoot Blight (TCSB) in Peach Caused by Diaporthe amygdali in the North of Italy in Emilia-Romagna

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Peach trees in northern Italy are suffering from a disease called twig canker and shoot blight caused by a fungus called Diaporthe amygdali. This research identified and characterized this fungus from affected orchards, studying how it grows at different temperatures to better understand and control the disease. The fungus grows best around 23-24°C and can survive extreme heat above 50°C. These findings will help fruit growers develop better strategies to protect their peach crops.

Research Keyword: ITS region