Research Keyword: housekeeping genes

Act1 out of Action: Identifying Reliable Reference Genes in Trichoderma reesei for Gene Expression Analysis

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Scientists studying the fungus Trichoderma reesei, which is used industrially to produce enzymes, found that the reference genes commonly used to measure gene activity (act1 and sar1) are not as reliable as previously thought. By analyzing genetic data from multiple studies, they identified two better reference genes called bzp1 and tpc1 that maintain more consistent expression levels across different growth conditions. Using these better reference genes will help researchers more accurately measure how genes are expressed in this important industrial fungus.

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Multilocus sequence typing of Candida albicans isolates from wild and farm animals from southern Italy

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Researchers in Italy studied a fungus called Candida albicans that can infect both humans and animals. They analyzed samples from chickens, wild boars, and lizards to understand how genetically similar these animal strains are to human infections. They found that animal strains are very similar to human strains, suggesting the fungus could be passed between animals and people. This research highlights the importance of monitoring fungal infections in animals to understand public health risks.

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Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR

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This research identifies the best internal control genes to use when measuring gene activity in a fungus that causes pepper disease. Scientists tested eight different reference genes under various conditions like fungal growth and pepper infection, plus when treating the fungus with plant extracts. Different genes worked best for different situations: one gene was best during fungal growth, another during infection, and a third when treated with plant extracts. These findings will help future studies better understand how this disease-causing fungus works and develop better control methods.

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Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions

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Scientists studied the Chinese caterpillar mushroom (Ophiocordyceps sinensis) to find the best genes to use as reference points when measuring other gene activity. They tested sixteen potential reference genes under various stress conditions and growth stages using a technique called RT-qPCR. They found that two specific genes (18S rRNA and beta-Tubulin) were the most reliable to use as standards, which will help researchers accurately study how this medicinal mushroom responds to different conditions.

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Multilocus sequence typing of Candida albicans isolates from wild and farm animals from southern Italy

mycolabadmin

Researchers studied a fungal pathogen called Candida albicans found in farm and wild animals in Italy to understand how it spreads between animals and humans. They used genetic analysis to compare isolates from laying hens, wild boars, and lizards with samples from infected humans around the world. The results showed that animal isolates were genetically similar to human clinical samples, suggesting animals could serve as reservoirs for this infection. This research highlights the importance of monitoring fungal diseases in animal populations as part of understanding disease transmission between animals and people.

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Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR

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Researchers identified the best internal control genes for measuring fungal gene expression in Colletotrichum scovillei, a fungus that causes serious disease in chili peppers. Different genes worked best under different conditions: one for normal growth, another during infection, and a third when treated with plant extracts. This discovery will help scientists study how this fungus develops and causes disease, potentially leading to better ways to control it using natural plant-based treatments.

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