Research Keyword: high-resolution melting analysis

Barcode high-resolution melting (Bar-HRM) analysis to authenticate true cinnamon (Cinnamomum verum) from its adulterants and contaminants

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This study developed a rapid and cost-effective DNA test to verify that cinnamon products are authentic true cinnamon (Ceylon cinnamon) rather than cheaper substitutes. The test uses DNA barcoding and melting curve analysis to distinguish true cinnamon from three common adulterants and can also detect contamination with a toxic fungus. The method is particularly useful for processed cinnamon products like powder where traditional identification methods don’t work, helping protect consumers and maintain market integrity for authentic Sri Lankan cinnamon.

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Dual-Emission FRET-PCR Outperforms SYBR Green and EvaGreen for Accurate Discrimination of Primary Canine Dermatophytes: Microsporum canis, Nannizzia gypsea, and Trichophyton mentagrophytes

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This research developed a new rapid test called FRET-PCR that can identify three common fungal infections in dogs within 2.5 hours, compared to 2-4 weeks for traditional culture methods. The test works by detecting specific genetic markers in fungal DNA and measuring temperature changes that differ for each fungal species. When compared to two other molecular tests (SYBR Green and EvaGreen), the FRET-PCR method was more accurate and sensitive, even able to detect a single copy of the target gene. This faster, more accurate identification will help veterinarians choose the right treatment more quickly and prevent spread of fungal infections.

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