Research Keyword: GFP tagging

Development of Green Fluorescent Protein-Tagged Strains of Fusarium acuminatum via PEG-Mediated Genetic Transformation

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Scientists developed a method to genetically modify a harmful fungus called Fusarium acuminatum that causes root rot in plants like carnations. Using a technique that breaks down the fungal cell wall and uses a special chemical (PEG) to insert genes, they successfully added a green-glowing protein (GFP) marker to the fungus. This allows researchers to track where and how the fungus infects plants. The modified fungus still behaves normally, making it a useful tool for identifying which genes make the fungus dangerous, potentially leading to better disease control methods.

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Histological Dissection of Fusarium-Banana Interaction Using a GFP-Tagged Subtropical Race 4 Strain of Fusarium oxysporum f. sp. cubense on Banana Cultivars with Differing Levels of Resistance

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Researchers used fluorescently-labeled fungal strains to visualize how banana wilt disease spreads inside banana plants. They found that resistant banana varieties can slow down the fungus by forming blockages (called tyloses) in their water-conducting vessels, though the fungus can still initially enter the plant. The study showed that the rhizome, an underground stem-like structure, is the key location where resistant plants successfully contain the fungus, which helps explain why some banana varieties are naturally more resistant to this devastating disease.

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PEG-Mediated Protoplast Transformation of Penicillium sclerotiorum (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

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Scientists developed a new method to genetically modify a fungus called Penicillium sclerotiorum by using protoplasts, which are fungal cells with their protective outer walls removed. They added a glowing green protein (GFP) to track the fungus and discovered that this modification changed how the fungus uses fats and lipids. When they treated tomato seeds with enzymes before exposing them to the modified fungus, it enhanced the fungus’s ability to colonize plant roots, potentially helping plants grow better.

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