genome assembly – Page 2

Gapless near Telomer-to-Telomer Assembly of Neurospora intermedia, Aspergillus oryzae, and Trichoderma asperellum from Nanopore Simplex Reads

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Scientists developed an automated computer workflow that can assemble complete fungal genomes using data from a single type of DNA sequencer. They tested this method on three industrially important fungi and successfully created high-quality, gap-free genome maps for all three. This breakthrough means researchers can now generate high-quality fungal genome sequences faster and more cheaply than before, which will help improve our understanding of these organisms.

Comparative genome analysis of patulin-producing Penicillium paneum OM1 isolated from pears

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Researchers sequenced the complete genome of a mold called Penicillium paneum that produces a toxic substance called patulin, which contaminates apples and pears. They found all 15 genes responsible for making patulin and discovered the mold has similar genetic patterns to other patulin-producing fungi. This information could help scientists develop better ways to prevent patulin contamination on fruit crops and improve food safety.

Multi-locus phylogenetic network analysis of Ampelomyces mycoparasites isolated from diverse powdery mildews in Australia and the generation of two de novo genome assemblies

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Scientists discovered and studied 20 new species of a beneficial fungus called Ampelomyces that naturally attacks powdery mildew, a common plant disease found in Australia. They created detailed genetic maps of two of these fungal strains to better understand how they work as biological control agents. This research could help develop better natural ways to protect crops like grapes and vegetables from powdery mildew without using chemical pesticides.

The genome sequence of the Oak Polypore, Buglossoporus quercinus (Schrad.) Kotl. & Pouzar

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Scientists have completed a full genetic map of the oak polypore, a rare and threatened mushroom that only grows on ancient oak trees. This mushroom is protected by law in the UK because it is becoming increasingly rare due to habitat loss and isolation. The detailed genetic blueprint will help scientists develop better strategies to protect and restore populations of this important forest fungus, potentially through carefully planned translocation programs.

Haplotype-Phased Chromosome-Level Genome Assembly of Floccularia luteovirens Provides Insights into Its Taxonomy, Adaptive Evolution, and Biosynthetic Potential

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Scientists successfully decoded the complete genetic blueprint of the yellow mushroom (Floccularia luteovirens), a valuable medicinal fungus found on the Tibetan Plateau. The high-quality genome assembly revealed the mushroom produces many different beneficial compounds like antitumor and anti-inflammatory molecules. The study also corrected previous scientific confusion about the mushroom’s evolutionary classification, showing it’s more closely related to other fungi than previously thought, and revealed how it adapted to harsh alpine conditions.

De novo Genome Assembly and Annotation of 12 Fungi Associated with Fruit Tree Decline Syndrome in ON, Canada

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Apple and stone fruit trees in Ontario have been dying at alarming rates since 2016. Researchers sequenced and analyzed the genetic code of 12 different fungi suspected of causing this decline. This study provides the first complete genetic blueprints for several fungal species, helping farmers and scientists better understand what causes the disease and develop ways to prevent it.

Draft genome sequence of three Glaciozyma watsonii strains isolated from near the Syowa station area, East Antarctica

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Scientists have sequenced the genetic code of three strains of a special cold-loving yeast called Glaciozyma watsonii found in East Antarctica. This yeast can grow in extremely cold conditions, even at temperatures below freezing, which is remarkable. Understanding its genome may help scientists develop new medicines and discover enzymes that work in cold conditions.

Haplotype-resolved genomes of Phlebopus portentosus reveal nuclear differentiation, TE-mediated variation, and saprotrophic potential

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Scientists sequenced the complete genomes of two compatible strains of the king bolete mushroom (Phlebopus portentosus), an important edible and medicinal species. The study found that mobile DNA elements called transposons play a major role in creating genetic differences between the two fungal nuclei and in generating the diversity of compounds that give mushrooms their health benefits. The research shows this mushroom can both partner with trees and break down organic material on its own, making it uniquely adaptable.

Genome assemblies for Pyricularia species and related genera isolated from diverse host plants

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Scientists have sequenced the complete genetic blueprints of 35 fungal strains belonging to Pyricularia species and related genera, which cause devastating blast diseases in crops like rice and wheat. By analyzing genetic differences between strains that infect different grass species, researchers found that most Pyricularia oryzae strains show strong preferences for specific host plants. These new genome resources will help scientists understand how fungal pathogens evolve and adapt to new plant hosts, potentially improving disease management strategies.

Genome sequence of the novel Cystobasidiomycetes fungal isolate EMM_F5

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Researchers isolated and sequenced the genome of a previously unclassified yeast called EMM_F5 found on Magnolia tree leaves. Through genetic analysis, they determined this yeast belongs to the Cystobasidiomycetes class and likely represents a new family called Microsporomycetaceae. This discovery fills an important gap in fungal genomics by providing the first genetic information available for this family of fungi.

Research Keyword: genome assembly