Research Keyword: genetic transformation

Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker

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Scientists have successfully developed a genetic engineering method for Madurella mycetomatis, the fungus that causes mycetoma, a serious tropical disease. They used a technique to remove the fungal cell wall and insert genes into the cells, creating strains that produce green fluorescent protein (GFP). This breakthrough enables researchers to better understand how this fungus causes disease and to develop new treatments.

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The Transformation and Protein Expression of the Edible Mushroom Stropharia rugosoannulata Protoplasts by Agrobacterium-tumefaciens-Mediated Transformation

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Researchers successfully developed a method to genetically modify the edible mushroom Stropharia rugosoannulata using Agrobacterium tumefaciens bacteria. This technique allows scientists to insert and express foreign genes in the mushroom, providing tools to study how specific genes control mushroom growth and the production of health-promoting compounds. The study demonstrates that both artificial and natural resistance markers can be used to identify successfully transformed mushrooms, offering a foundation for improving mushroom cultivation and breeding.

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Expression and antiviral application of exogenous lectin (griffithsin) in sweetpotatoes

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Scientists successfully created sweetpotato plants that produce griffithsin, a powerful antiviral protein found in marine algae. When these modified plants were grown in fields, they resisted a devastating sweetpotato virus disease that normally destroys crops. Interestingly, the virus-fighting mechanism works differently in plants than in animals—instead of directly blocking the virus, griffithsin triggers the plant’s own natural antiviral defense genes.

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