genetic transformation

The Transformation and Protein Expression of the Edible Mushroom Stropharia rugosoannulata Protoplasts by Agrobacterium-tumefaciens-Mediated Transformation

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Scientists developed a genetic engineering technique to modify king stropharia (a cultivated edible mushroom) by inserting foreign genes into its cells. This breakthrough allows researchers to study how the mushroom grows and produces beneficial compounds. The technique uses a bacterium called Agrobacterium tumefaciens to naturally deliver genes into mushroom cells, similar to how it infects plants. This advancement could lead to improved cultivation practices and enhanced nutritional or medicinal properties.

Establishment of a genetic transformation system for cordycipitoid fungus Cordyceps chanhua

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Scientists have successfully developed a method to genetically modify Cordyceps chanhua, an important medicinal mushroom used in traditional Chinese medicine for over 1,600 years. By optimizing how they prepare fungal cells and introduce foreign genes, they created a reliable genetic transformation system that can be used to study which genes produce beneficial compounds in this mushroom. This breakthrough will help researchers understand and potentially enhance the medicinal properties of C. chanhua.

Edible mushrooms as emerging biofactories for natural therapeutics and oral biopharmaceutical delivery

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Mushrooms are emerging as natural medicine factories that can be genetically engineered to produce medications taken by mouth. Beyond their traditional use as health foods, scientists are now using advanced genetic techniques to program mushrooms to manufacture therapeutic proteins and vaccines. These engineered mushrooms can naturally package and protect these medications as they pass through the stomach, releasing them safely in the intestines for absorption. This approach offers a sustainable, affordable, and cold-chain-independent alternative to conventional injected medications.

Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in Cordyceps cicadae

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Scientists successfully developed a method to genetically modify Cordyceps cicadae, a valuable medicinal fungus used in traditional Chinese medicine. By optimizing how to remove the fungus’s protective cell wall and introducing new genes using a technique called PEG-mediated transformation, researchers created a stable system for genetic manipulation. This breakthrough opens the door for improving medicinal compounds in this fungus and advancing its use in treating various health conditions.

The Potential of Transgenic Hybrid Aspen Plants with a Recombinant Lac Gene from the Fungus Trametes hirsuta to Degrade Trichlorophenol

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Scientists created genetically modified aspen trees that contain an enzyme from a fungus which breaks down toxic chlorophenol chemicals in soil. These transgenic trees were much more effective at removing these harmful pollutants than regular trees. Three of the modified tree lines worked well without causing problems for the plants themselves, suggesting they could be used to clean up contaminated soil in polluted areas.

Strategy of employing plug-and-play vectors and LC–MS screening to facilitate the discovery of natural products using Aspergillus oryzae

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Researchers developed new tools to make it faster and easier to discover useful compounds from fungi. They created improved genetic vectors that allow scientists to insert multiple genes into Aspergillus oryzae more conveniently, and developed a quick screening method using mass spectrometry to identify successful transformants directly on culture plates. This approach saves about 10 days compared to traditional methods, significantly accelerating the discovery of new natural products with potential medical and agricultural applications.

Saprotrophic Arachnopeziza Species as New Resources to Study the Obligate Biotrophic Lifestyle of Powdery Mildew Fungi

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Scientists have created a new way to study powdery mildew fungus, which causes widespread plant disease but cannot normally be studied in the laboratory. They identified a related fungus species (Arachnopeziza) that can grow in culture and can be genetically modified. By sequencing the DNA of these two Arachnopeziza species and developing methods to alter their genes, researchers have created a practical tool to understand how powdery mildew becomes dependent on its plant host, potentially leading to better disease control strategies.

Expression of a novel NaD1 recombinant antimicrobial peptide enhances antifungal and insecticidal activities

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Scientists created a new genetically modified tobacco plant that produces a powerful natural pest-fighting protein called NaD1. By attaching special chitin-binding components to this protein, they made it stick better to fungal pathogens and insect digestive systems. When tested, these enhanced proteins killed fungi more effectively and caused higher mortality rates in crop-damaging insects, offering a promising natural alternative to chemical pesticides.

PEG-Mediated Protoplast Transformation of Penicillium sclerotiorum (scaumcx01): Metabolomic Shifts and Root Colonization Dynamics

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Scientists developed a new method to genetically modify a beneficial fungus called Penicillium sclerotiorum by removing its cell wall and introducing new genes. They added a glowing green marker (GFP) to track the fungus as it colonizes tomato plant roots. The study shows that enzymatic treatment of seeds significantly improves how well the fungus attaches to roots, potentially helping plants grow better while revealing how the genetic modification affects the fungus’s internal chemistry.

Construction of a heat-resistant strain of Lentinus edodes by fungal Hsp20 protein overexpression and genetic transformation

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Scientists successfully created a heat-resistant version of shiitake mushrooms by adding extra copies of a heat-protection gene from button mushrooms. The modified mushrooms can survive higher temperatures and recover better after heat stress compared to regular shiitake strains. This genetic improvement could help shiitake farming expand to warmer regions and times of year, potentially increasing production worldwide.

Research Keyword: genetic transformation