Research Keyword: gene expression

Multi-omics analysis of Taiwanofungus gaoligongensis: effects of different cultivation methods on secondary metabolites

This study examined how different growing methods affect the medicinal compounds found in Taiwanofungus gaoligongensis, a rare Chinese medicinal fungus. Researchers discovered that growing this fungus on wood substrates from specific trees significantly increased production of beneficial compounds like antcins and antrodin C, which have anti-cancer and anti-inflammatory properties. By analyzing gene expression patterns, they identified key genes and regulatory mechanisms that control the production of these medicinal compounds, suggesting ways to improve cultivation methods for better medicinal value.

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Transcriptome analysis of Ochratoxin A (OTA) producing Aspergillus westerdijkiae fc-1 under varying osmotic pressure

Researchers studied how salt levels affect the production of Ochratoxin A (OTA), a harmful toxin made by a fungus commonly found in foods like coffee and dried meats. Using genetic analysis techniques, they found that different salt concentrations trigger different genes in the fungus, affecting how much toxin it produces. This research helps explain why OTA contamination is worse in high-salt foods and could lead to better ways to prevent food poisoning from this fungus.

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Cloning and Expression Analysis of Phenylalanine Ammonia-Lyase Gene in the Mycelium and Fruit Body of the Edible Mushroom Flammulina velutipes

Scientists cloned and studied a gene called PAL in the winter mushroom (Flammulina velutipes), which produces trans-cinnamic acid from phenylalanine. They found that this gene is activated differently depending on the nutrient environment and mushroom developmental stage. The gene is particularly active in the mushroom’s stem during growth, suggesting it helps produce beneficial compounds during mushroom development.

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Effects and molecular mechanism of endophytic elicitors on the accumulation of secondary metabolites in medicinal plants

This review explains how beneficial fungi living inside medicinal plants can boost the production of healing compounds. These endophytic fungi act as natural triggers that activate the plant’s own defense systems, causing it to produce more of the valuable medicinal substances used in traditional and modern medicine. By understanding how this process works, scientists can develop better methods to grow medicinal plants and produce natural drugs more sustainably without depleting wild plant populations.

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Transcriptome Analysis Explored the Differential Genes’ Expression During the Development of the Stropharia rugosoannulata Fruiting Body

Researchers studied how S. rugosoannulata mushrooms grow and develop by analyzing which genes are active at different stages of fruit body formation. They found that the mushroom’s development relies heavily on glucose and amino acid metabolism, with special genetic processes called alternative splicing playing key roles in maturation. This is the first comprehensive genetic study of this edible mushroom’s development, providing valuable information for improving cultivation techniques and mushroom quality.

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Analysis of the chitin synthase gene family in Ganoderma lucidum: its structure, phylogeny, and expression patterns

Scientists studied eight chitin synthase genes in Ganoderma lucidum (reishi mushroom), which are important for building the fungal cell wall and controlling growth. They found that different genes are active at different stages of mushroom development and that six of these genes become more active when exposed to high temperatures, suggesting they help the mushroom survive heat stress. This research helps us understand how mushrooms grow and develop, which could lead to better cultivation methods.

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Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR

This research identifies the best internal control genes to use when measuring gene activity in a fungus that causes pepper disease. Scientists tested eight different reference genes under various conditions like fungal growth and pepper infection, plus when treating the fungus with plant extracts. Different genes worked best for different situations: one gene was best during fungal growth, another during infection, and a third when treated with plant extracts. These findings will help future studies better understand how this disease-causing fungus works and develop better control methods.

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Occurrence and function of enzymes for lignocellulose degradation in commercial Agaricus bisporus cultivation

White button mushrooms grow on compost made from straw and manure through carefully controlled phases. The mushroom uses specific enzymes to break down tough plant materials, especially lignin during the growing mycelium stage, which helps make nutrients available for mushroom formation. Understanding these enzymatic processes could help mushroom farmers improve their production by developing better mushroom strains that degrade plant materials more efficiently.

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Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions

Scientists studied the Chinese caterpillar mushroom (Ophiocordyceps sinensis) to find the best genes to use as reference points when measuring other gene activity. They tested sixteen potential reference genes under various stress conditions and growth stages using a technique called RT-qPCR. They found that two specific genes (18S rRNA and beta-Tubulin) were the most reliable to use as standards, which will help researchers accurately study how this medicinal mushroom responds to different conditions.

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