gene expression – Page 3

Act1 out of Action: Identifying Reliable Reference Genes in Trichoderma reesei for Gene Expression Analysis

mycolabadmin

Scientists studying the fungus Trichoderma reesei, which is used industrially to produce enzymes, found that the reference genes commonly used to measure gene activity (act1 and sar1) are not as reliable as previously thought. By analyzing genetic data from multiple studies, they identified two better reference genes called bzp1 and tpc1 that maintain more consistent expression levels across different growth conditions. Using these better reference genes will help researchers more accurately measure how genes are expressed in this important industrial fungus.

Genome-wide identification and transcriptome analysis of the cytochrome P450 genes revealed its potential role in the growth of Flammulina filiformis

mycolabadmin

Scientists identified 59 cytochrome P450 genes in the golden needle mushroom (Flammulina filiformis), an economically important edible fungus. These genes appear to control the mushroom’s growth and development, particularly the elongation of the stalk. By understanding how these genes work, researchers can potentially improve mushroom cultivation and develop new varieties with better growth characteristics. This research provides valuable insights into the genetics of mushroom growth and development.

Chromosome-Scale Genome and Transcriptomic Analyses Reveal Differential Regulation of Terpenoid Secondary Metabolites in Hericium coralloides

mycolabadmin

Researchers sequenced the complete genome of Hericium coralloides, an edible medicinal mushroom, at the chromosome level for the first time. They identified genes responsible for producing terpenoids, which are beneficial compounds with antioxidant, anti-inflammatory, and anti-tumor properties. The study found that these beneficial compounds are produced in higher amounts in the mushroom’s mycelium (root-like structure) compared to fruiting bodies, which could help optimize mushroom cultivation for medicinal use.

Revitalization of the Endophytic Fungus Acremonium sp. MEP2000 and Its Impact on the Growth and Accumulation of Bioactive Compounds in Inonotus obliquus

mycolabadmin

Researchers successfully revived a beneficial fungus called Acremonium sp. MEP2000 that had lost its effectiveness through repeated cultivation by adding birch bark powder and medicinal mushroom powder to its growth medium. When used to treat the medicinal fungus Inonotus obliquus (chaga), this revitalized fungal culture dramatically improved the growth and production of healthy bioactive compounds like polysaccharides and triterpenoids. This breakthrough offers a practical solution for large-scale production of medicinal fungi with enhanced therapeutic potential for treating cancer, diabetes, and inflammatory conditions.

Immunomodulatory Effects of Edible and Medicinal Mushrooms and Their Bioactive Immunoregulatory Products

mycolabadmin

Mushrooms have been used for health for thousands of years and contain special compounds that help boost the immune system and fight cancer. Scientists have identified several key active ingredients in mushrooms like polysaccharides and proteins that can activate immune cells and reduce tumors. Some mushroom products like lentinan from shiitake mushrooms have already been approved as cancer treatments in Japan. While most evidence comes from lab studies, ongoing research suggests mushroom-based medicines could become important therapeutic options.

Genome-wide analysis of bZIP gene family members in Pleurotus ostreatus, and potential roles of PobZIP3 in development and the heat stress response

mycolabadmin

Scientists identified 11 genes that code for special proteins called bZIP transcription factors in oyster mushrooms. One particular protein, PobZIP3, was found to help mushrooms survive high temperatures and grow faster. When researchers increased this protein in mushroom strains, the mushrooms became more heat-resistant and produced edible fruiting bodies more quickly, suggesting this discovery could help farmers grow oyster mushrooms more reliably.

The biocontrol potential of endophyte Bacillus velezensis to reduce post-harvest tomato infection caused by Rhizopus microsporus

mycolabadmin

Researchers tested a naturally occurring bacteria called Bacillus velezensis as a safe alternative to chemical fungicides for protecting tomatoes from rot-causing mold. The bacteria produces special compounds that can kill or slow down the growth of Rhizopus microsporus, a fungus that commonly spoils tomatoes after harvest. While the results show promise, the effectiveness varied depending on which specific strain of bacteria and mold was used, suggesting more research is needed to fine-tune the approach.

Comparative Transcriptome Profiles of the Response of Mycelia of the Genus Morchella to Temperature Stress: An Examination of Potential Resistance Mechanisms

mycolabadmin

Scientists studied how morel mushrooms respond to different temperatures to understand why cultivation can be unpredictable. By analyzing gene activity in mushroom mycelia (the underground filaments) at temperatures from 5°C to 30°C, they found that 15-20°C was ideal for growth. At higher temperatures, the mushrooms showed signs of stress similar to heat damage in other organisms, turning brownish and activating protective genes. This research helps mushroom farmers optimize growing conditions for better yields.

Genome-Wide Characterization and Expression Profiling of Phytosulfokine Receptor Genes (PSKRs) in Triticum aestivum with Docking Simulations of Their Interactions with Phytosulfokine (PSK): A Bioinformatics Study

mycolabadmin

This study mapped and analyzed receptor genes in wheat that respond to a natural plant hormone called phytosulfokine. Researchers identified 57 versions of these receptor genes distributed across wheat’s genome and found they are most active in roots and leaves at different growth stages. Computer modeling showed how the plant hormone binds to its receptors. These findings could help develop wheat varieties that grow better and handle stress more effectively.

Context-Dependent Fitness Trade-Offs in Penicillium expansum Isolates Resistant to Multiple Postharvest Fungicides

mycolabadmin

This research examines how apples and pears get a fungal disease called blue mold and how the fungus develops resistance to commonly used fungicides. Scientists tested fungus samples that resist different fungicides to see if this resistance makes them weaker. They found that resistant fungus strains do struggle more under stressful laboratory conditions, but remain dangerous during long-term cold storage of fruit, especially when fungicides are present.

Research Keyword: gene expression