Research Keyword: fungal pathogen detection

Optimizing a modified cetyltrimethylammonium bromide protocol for fungal DNA extraction: Insights from multilocus gene amplification

This research improves how scientists extract DNA from fungi found in dried fruits and other sources. The modified CTAB method is faster, cheaper, and produces higher quality DNA than commercial kits, without requiring special equipment like liquid nitrogen. The extracted DNA works well for identifying fungal species and detecting fungal diseases in fish, plants, and humans, making it valuable for both research and medical diagnostics.

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Detection of Pestalotiopsis abbreviata sp. nov., the Causal Agent of Pestalotiopsis Leaf Blight on Camellia japonica Based on Metagenomic Analysis

Researchers discovered a new fungal species called Pestalotiopsis abbreviata that causes leaf disease on Camellia japonica, an ornamental tree popular in Korea. Instead of using traditional time-consuming laboratory methods, they used advanced genetic sequencing (metagenomic analysis) to quickly identify the fungus among hundreds of other microbes living on the tree leaves. This new approach is faster and more accurate than old methods, making it easier to monitor and manage plant diseases in the future.

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