Research Keyword: fungal genomics

Contamination of fungal genomes of Onygenaceae (Phylum Ascomycota) in public databases: incidence, detection, and impact

Scientists found that many fungal genome sequences stored in public databases contain unwanted bacterial DNA that can interfere with research results. They developed a method to identify and remove this contamination using related high-quality fungal genomes as reference. After cleaning four contaminated genomes, the quality improved significantly and the contamination dropped from 5-12% to below 3%, demonstrating that careful screening is essential for reliable genetic research.

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Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees

Scientists developed an optimized method for growing brown spot needle blight fungi from infected pine needles and extracting their DNA for research. They tested four different growth media and four DNA extraction techniques to find the best combination. Sabouraud dextrose medium combined with a CTAB-based extraction method containing polyvinylpyrrolidone worked best, producing high-quality DNA suitable for advanced genetic studies. This standardized approach will help researchers better understand this important forest disease.

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Diverse nitrogen acquisition strategies of conifer-associated ectomycorrhizal fungi shape unique responses to changing nitrogen regimes

This research examines how different types of mushroom fungi that partner with conifer trees acquire nitrogen in different ways. Using genomic analysis, lab experiments, and historical mushroom samples from the past 60 years, scientists found that even closely related fungal species have distinct strategies for obtaining nitrogen from different sources. These findings suggest that coniferous forests may respond quite differently to changes in nitrogen availability compared to forests with broadleaf trees.

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Myco-Ed: Mycological curriculum for education and discovery

Myco-Ed is an educational program that teaches students about fungi while helping scientists discover new fungal species. Students collect fungi from their environment, learn laboratory and computing skills, and send samples to be sequenced to create reference genomes. This program both trains the next generation of fungal experts and expands our understanding of fungal diversity, which is important for medicine, agriculture, and the environment.

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Complete mitochondrial genome of the ectomycorrhizal fungus Suillus collinitus (Suillaceae, Boletales)

Scientists have successfully sequenced the complete genetic code of a mushroom species called Suillus collinitus, which forms important partnerships with pine trees. This mushroom’s mitochondrial genome is about 77,700 base pairs long and contains genes necessary for cellular energy production. The study reveals that this species belongs to the Boletales group of fungi and provides valuable genetic information that can help researchers better understand fungal evolution and relationships.

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Whole-genome sequencing of Fusarium oxysporum K326-S isolated from tobacco

Scientists have sequenced the complete genetic blueprint of a fungus that causes root rot in tobacco plants. This fungus, Fusarium oxysporum K326-S, damages tobacco crops by causing roots to brown and wilt. The detailed genome map they created contains over 17,000 genes and will help farmers and researchers develop better strategies to prevent and control this destructive disease in the future.

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