Research Keyword: freeze-thaw cycles

Cellulose-Based Hydrogels for Wastewater Treatment: A Focus on Metal Ions Removal

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Heavy metal pollution from industrial activities poses serious health risks including cancer, kidney damage, and neurological problems. This review explores how cellulose-based hydrogels—soft, water-absorbing materials made from natural plant sources—can effectively remove toxic metals from contaminated water. These hydrogels are cost-effective, environmentally friendly, and can be reused multiple times, making them promising alternatives to conventional water treatment methods for industrial and municipal applications.

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The Effects of Iterative Freeze–Thaw Cycles on the Structure, Functionality, and Digestibility of Grifola frondosa Protein

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This research shows that freezing and thawing maitake mushroom protein in controlled cycles can significantly improve its ability to stabilize oil and water mixtures in food products. The best results occur after 2-3 cycles, which enhance both how well the protein breaks down during digestion and how effectively it works as an emulsifier in foods like sauces and dressings. This simple physical treatment method offers a sustainable way to improve mushroom protein for food manufacturing without affecting its nutritional quality.

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Time- and temperature-dependent Pentraxin 3 stability in serum and bronchoalveolar lavage fluid samples

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Pentraxin 3 is a protein marker that helps doctors detect serious fungal infections. This study tested how well this marker stays stable in blood and lung fluid samples when stored at different temperatures over long periods. The results show that samples should be kept frozen at −20°C or −80°C to preserve the marker, but samples left at room temperature or warmer are quickly damaged and unreliable for testing.

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Time- and temperature-dependent Pentraxin 3 stability in serum and bronchoalveolar lavage fluid samples

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Researchers tested how stable Pentraxin 3, a protein marker for fungal infections, remains when stored under different conditions. They found that samples stored in freezers at −20°C or −80°C kept stable for months, making Pentraxin 3 a reliable marker for diagnosing serious fungal infections. However, samples stored at warm temperatures degraded quickly, so proper cold storage is essential for accurate test results.

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Effects of Freeze–Thaw Cycles on the Structures and Functional Properties of Clitocybe squamulosa Protein Isolates

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Researchers studied how repeatedly freezing and thawing a protein extract from the edible Clitocybe squamulosa mushroom affects its usefulness in food products. They found that three freeze-thaw cycles improved the protein’s ability to create stable foams and emulsions, while two cycles best preserved digestibility and antioxidant benefits. This simple, chemical-free treatment method could help food manufacturers create better products using mushroom proteins.

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