Research Keyword: food authentication

Multiple Technology Approach Based on Stable Isotope Ratio Analysis, Fourier Transform Infrared Spectrometry and Thermogravimetric Analysis to Ensure the Fungal Origin of the Chitosan

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This study develops reliable methods to identify whether chitosan used in winemaking comes from mushrooms or shellfish. Using isotope analysis, heat testing, and infrared spectroscopy, researchers established clear markers to distinguish fungal chitosan from crustacean-derived versions. This is important because only mushroom-derived chitosan is legally permitted in wine production due to allergy risks associated with shellfish proteins.

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Morphological, Genetic, and Microbiological Characterization of Tuber magnatum Picco Populations from Alto Molise, Central-Southern Italy

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This research studied Italian white truffles from the Molise region, one of Italy’s most important truffle-producing areas. Scientists examined 20 truffles from four different locations, analyzing their physical characteristics, genetic makeup, and the bacteria and fungi living inside them. They discovered a unique genetic pattern found only in Molise truffles and identified complex microbial communities that may help authenticate where truffles came from, helping prevent food fraud and protect truffle producers.

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Fatty Acids and Stable Isotope Ratios in Shiitake Mushrooms (Lentinula edodes) Indicate the Origin of the Cultivation Substrate Used: A Preliminary Case Study in Korea

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Shiitake mushrooms are commonly grown in Korea using substrates from both Korea and China, but consumers often cannot tell which origin was used. This study compared mushrooms grown on Korean versus Chinese substrates and found that measuring stable isotope ratios (chemical signatures) is more reliable than analyzing fatty acids for determining where the cultivation substrate came from. These results could help improve labeling accuracy in the mushroom market.

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Barcode high-resolution melting (Bar-HRM) analysis to authenticate true cinnamon (Cinnamomum verum) from its adulterants and contaminants

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This study developed a rapid and cost-effective DNA test to verify that cinnamon products are authentic true cinnamon (Ceylon cinnamon) rather than cheaper substitutes. The test uses DNA barcoding and melting curve analysis to distinguish true cinnamon from three common adulterants and can also detect contamination with a toxic fungus. The method is particularly useful for processed cinnamon products like powder where traditional identification methods don’t work, helping protect consumers and maintain market integrity for authentic Sri Lankan cinnamon.

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Identification of matB used as an endogenous reference gene for the qualitative and real-time quantitative polymerase chain reaction detection of Lentinus edodes

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This study identifies the matB gene as a reliable genetic marker for detecting shiitake mushrooms (Lentinus edodes) in food products. Researchers developed a simple DNA test that can identify L. edodes in processed foods where it might be fraudulently substituted for more expensive wild mushrooms. The test is highly sensitive, detecting DNA at extremely low concentrations, and works on both raw mushrooms and processed products. This method provides food manufacturers and regulators with an effective tool to prevent mushroom fraud and ensure food authenticity.

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