fluorescence microscopy

Screening of a Fraction with Higher Amyloid β Aggregation Inhibitory Activity from a Library Containing 210 Mushroom Extracts Using a Microliter-Scale High-Throughput Screening System with Quantum Dot Imaging

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Researchers screened 210 mushroom species from Japan to find those that could prevent harmful protein clumping in the brain associated with Alzheimer’s disease. They identified a purified fraction from the mushroom Elfvingia applanata that was more effective than a known anti-Alzheimer’s compound at stopping these protein clumps from forming. The extract also protected nerve cells from damage in laboratory tests. This finding suggests mushrooms could potentially be developed into a functional food to help prevent Alzheimer’s disease.

Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis

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Scientists studied mushroom cells to understand structures called lomasomes that form at the cell membrane surface. Using advanced microscopy techniques, they discovered these structures are made of tiny tubes and layers that can fold and swell into different shapes. These findings help explain how fungal cells that decompose wood organize their membranes and may be involved in how cells take in materials from their surroundings.

Characterization of ORF19.7608 (PPP1), a biofilm-induced gene of Candida albicans

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Researchers studied a gene called PPP1 in Candida albicans, a common fungal infection in hospitals. They found that this gene is highly active when the fungus forms protective biofilms on medical devices like catheters. Although the protein appears in a distinctive spotted pattern only during biofilm formation, removing this gene did not prevent biofilm formation or affect how the fungus responds to stress or antifungal drugs.

A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi

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Scientists discovered that many fungi cannot be stained with phalloidin, a widely-used fluorescent dye that helps visualize actin filaments in cells. They traced this problem to a single amino acid difference in fungal actin proteins. By changing this one amino acid back to its original form using genetic engineering, they successfully enabled phalloidin staining in previously incompatible fungi, providing researchers with better tools to study fungal cell biology.

Research Keyword: fluorescence microscopy

Screening of a Fraction with Higher Amyloid β Aggregation Inhibitory Activity from a Library Containing 210 Mushroom Extracts Using a Microliter-Scale High-Throughput Screening System with Quantum Dot Imaging

Lomasomes and Other Fungal Plasma Membrane Macroinvaginations Have a Tubular and Lamellar Genesis

Characterization of ORF19.7608 (PPP1), a biofilm-induced gene of Candida albicans

A genetic strategy to allow detection of F-actin by phalloidin staining in diverse fungi