Research Keyword: enzyme kinetics

Aflatoxin B1 (AFB1) biodegradation by a lignolytic phenoloxidase of Trametes hirsuta

Scientists discovered that a mushroom called Trametes hirsuta produces a special enzyme that can break down aflatoxin B1, a dangerous toxin that contaminates foods like peanuts, corn, and nuts. This enzyme is unique because it works without needing additional chemicals as helpers, making it practical for real-world use. The enzyme successfully degraded 77.9% of the toxin under simple conditions, and researchers suggest it could be applied directly to contaminated food surfaces as a safe, natural way to reduce food poisoning risks.

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Applications of Microbial Organophosphate-Degrading Enzymes to Detoxification of Organophosphorous Compounds for Medical Countermeasures against Poisoning and Environmental Remediation

Organophosphate compounds are toxic chemicals used as pesticides and banned chemical weapons that pose serious health and environmental risks. Scientists have discovered and engineered special enzymes from bacteria and other microorganisms that can break down these toxic compounds. These enzymes can be used as medical treatments to protect people exposed to organophosphates or as environmental cleanup tools to decontaminate poisoned soil and water.

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Bibliometric analysis of global research on white rot fungi biotechnology for environmental application

White rot fungi are special mushrooms that can break down difficult-to-decompose pollutants in soil and water, offering a natural and cost-effective way to clean up environmental contamination. This research study analyzed over 3,900 scientific publications about using these fungi for environmental cleanup from 2003 to 2020. The analysis found that research on white rot fungi has grown significantly, with scientists from China and the USA leading the field, and identified three major application areas: treating biomass waste, removing dyes from wastewater, and cleaning polluted environments.

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White Rot Fungi as Tools for the Bioremediation of Xenobiotics: A Review

White rot fungi are nature’s cleanup crew that can break down many toxic chemicals in our environment, from industrial dyes to pesticides. These special fungi produce powerful enzymes that can degrade pollutants that normally resist breakdown, making them promising tools for cleaning contaminated soil and water. Scientists are studying how to better harness these fungi’s abilities to treat industrial wastewater and restore polluted environments.

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Exo- and Endo-1,5-α-L-Arabinanases and Prebiotic Arabino-Oligosaccharides Production

This review explores arabino-oligosaccharides (AOS), special sugar compounds that act as prebiotics to feed beneficial gut bacteria. Scientists use enzymes called arabinanases to produce these AOS from plant material, particularly from arabinan found in sugar beets. Different types of these enzymes create different AOS products with varying health benefits, making them promising ingredients for functional foods and supplements that support digestive health.

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Vermiculite as a new carrier for extracellular protease production by Aspergillus spp. under solid-state fermentation

Researchers discovered that vermiculite, a naturally occurring mineral, is an excellent material for growing fungi that produce proteases—enzymes used in medicine, laundry detergents, and food processing. When Aspergillus fungi were grown on vermiculite using a technique called solid-state fermentation, they produced 3 to 18 times more protease than when grown on other materials. This discovery could make enzyme production more efficient and cost-effective for industrial applications.

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If you cannot see it, is it still there? – Ensemble refinement of invisible protein segments

Scientists studying protein structures using X-ray crystallography often face a problem: flexible parts of proteins don’t show up clearly in their images and get left out of molecular models. This paper demonstrates a new technique called ensemble refinement that can visualize these ‘invisible’ flexible regions by creating multiple model versions simultaneously. Using a mushroom enzyme that produces psilocybin as an example, researchers show that this approach reveals how protein parts actually move and change shape, providing better understanding of how proteins function.

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Optimization of cultural conditions for pectinase production by Diaporthe isolate Z1-1N and its pathogenicity on kiwifruit

Researchers studied how a fungus called Diaporthe causes soft rot in kiwifruit by producing special enzymes called pectinases that break down the fruit’s cell walls. They found the best conditions for growing these enzymes in the lab: a temperature of 28°C, neutral pH around 7.5, and 2-3 days of growth. When they extracted these pure enzymes and put them on fresh kiwifruit, the enzymes caused damage equivalent to about half the damage caused by the living fungus itself, proving these enzymes are important for disease development.

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Efficient conversion of tea residue nutrients: Screening and proliferation of edible fungi

Researchers developed an environmentally friendly method to convert tea waste into nutritious fungal protein using edible mushrooms. By testing six different fungal species, they found that Monascus kaoliang B6 was most efficient at breaking down the complex fiber structures in tea residue and converting them into fungal biomass. This sustainable process eliminates the need for chemical treatments and harsh conditions, turning agricultural waste into valuable food ingredients.

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Antioxidant and Enzyme Inhibitory Potential of Streptomyces sp. G-18 Grown in Various Media

Researchers studied bacteria called Streptomyces that were isolated from high mountains in Nepal to see if they could produce useful compounds. They grew these bacteria in four different types of growth media and tested the resulting extracts for antioxidant properties and ability to block harmful enzymes. They found that the choice of growth medium significantly affected what compounds the bacteria produced, with one medium (R2YE) being especially effective at producing compounds that could help treat diseases like Alzheimer’s and diabetes.

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