Research Keyword: enzymatic digestion

Optimized protein extraction protocol from human skin samples

Researchers developed an improved method for extracting and analyzing proteins from human skin samples. Using specialized equipment and chemical treatments, they were able to identify about 6,000 different proteins in skin tissue, which is significantly more than previous methods could detect. This new protocol is particularly useful for studying skin diseases like fungal infections and could help identify new treatments by revealing how proteins change in diseased skin.

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Engineering Strategies for Fungal Cell Disruption in Biotechnological Applications

Fungi produce valuable products inside their cells like medicines, oils, and natural colors. However, fungal cell walls are very tough and hard to break open compared to bacteria or algae. Scientists have developed various methods to break open fungal cells, ranging from physical approaches like grinding with beads or using sound waves, to gentler chemical and enzymatic methods. The best method depends on the type of fungus, what product you want to extract, and how much you need to make.

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Improved Protoplast Production Protocol for Fungal Transformations Mediated by CRISPR/Cas9 in Botrytis cinerea Non-Sporulating Isolates

Scientists have developed a better method to isolate protoplasts (fungal cells without cell walls) from non-sporulating varieties of gray mold fungus. By optimizing the incubation time, culture container, and enzyme used, they produced more viable protoplasts that can regenerate and be genetically modified. This advancement allows researchers to use CRISPR gene-editing technology to understand and potentially control gray mold, which causes significant crop losses worldwide.

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