Research Keyword: Dothideomycetes

Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees

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Scientists developed an optimized method for growing brown spot needle blight fungi from infected pine needles and extracting their DNA for research. They tested four different growth media and four DNA extraction techniques to find the best combination. Sabouraud dextrose medium combined with a CTAB-based extraction method containing polyvinylpyrrolidone worked best, producing high-quality DNA suitable for advanced genetic studies. This standardized approach will help researchers better understand this important forest disease.

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Morpho-molecular analyses reveal two novel species and two new records of Dictyosporiaceae (Pleosporales) from Dulongjiang River in northwestern Yunnan Province, China

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Scientists discovered and identified four new or newly recorded fungal species from a river in China’s Yunnan Province. These fungi, belonging to the Dictyosporiaceae family, were found growing on submerged wood in the Dulongjiang River. By combining detailed microscopic observation with genetic analysis, researchers confirmed two completely new species and two species not previously recorded in this region, expanding our understanding of freshwater fungal diversity.

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Multigene phylogeny and taxonomy of Dendryphion hydei and Torula hydei spp. nov. from herbaceous litter in northern Thailand

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Scientists discovered and formally named two new fungal species from dead plant material in northern Thailand. Using both traditional microscopy to examine the fungi’s physical structures and modern DNA analysis, they determined these are previously unknown species within the Torulaceae fungal family. The research helps scientists better understand fungal diversity and how different fungal species are related to each other.

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