Research Keyword: disease screening

Standardization of challenge inoculation protocols for artificial infection of Fusarium acutatum and Fusarium falciforme causing basal rot of onion

This research develops standardized methods for infecting onion bulbs with fungal diseases in laboratory settings to test which onion varieties can resist the infection. Two Fusarium fungi species that cause onion basal rot were tested using five different inoculation techniques. The cotton swab method proved most effective and practical for screening large numbers of onion plants. These findings will help farmers and plant breeders develop onion varieties resistant to these economically important diseases.

Read More »

Flood Inoculation of Fusarium eumartii in Tomato Seedlings: Method for Evaluating the Infectivity of Pathogen Spores

Scientists have developed a simple laboratory method to test how effectively a fungus called Fusarium eumartii infects young tomato plants. The technique involves growing tomato seedlings on special nutrient plates and then exposing them to fungal spores suspended in water, allowing researchers to measure how much damage occurs and how much fungus remains in the plant tissue. This method can be used to quickly test whether different compounds, like chitosan, can prevent fungal infections or help plants defend themselves naturally. The straightforward approach makes it useful for farmers and researchers developing better ways to protect tomato crops from fungal diseases.

Read More »

A rapid and efficient in vivo inoculation method for introducing tree stem canker pathogens onto leaves: suitable for large-scale assessment of resistance in poplar breeding progeny

Scientists developed a faster and easier way to test whether poplar trees are resistant to stem canker diseases caused by fungi. Instead of using traditional time-consuming stem tests, they inoculate fungal pathogens directly onto leaves, which shows results in just 5 days. This new leaf-based method uses abundant, readily available leaf tissue and can test many poplar breeding candidates quickly and cheaply, making it ideal for developing disease-resistant poplar varieties.

Read More »
Scroll to Top