Research Keyword: dioxygenase

Computer-directed rational engineering of dioxygenase TcsAB for triclosan biodegradation under cold conditions

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Scientists engineered a special enzyme called TcsAB to work better at cold temperatures, enabling it to break down triclosan, a harmful antibacterial chemical that pollutes our water. By using computer simulations and strategic mutations, they created a modified enzyme that degrades triclosan 2.5 times more efficiently at 15°C. When inserted into bacteria, this engineered enzyme helps clean up triclosan pollution in natural environments without requiring energy-intensive heating.

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Screening, identification, metabolic pathway of di-n-butyl phthalate degrading Priestia megaterium P-7 isolated from long-term film mulched cotton field soil in Xinjiang

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This research identifies a special bacterium called Priestia megaterium P-7 that can efficiently break down di-n-butyl phthalate (DBP), a harmful plastic chemical that accumulates in cotton field soils. Scientists found that this bacterium can completely remove DBP from contaminated soil within 20 hours under optimal conditions. By studying the bacterium’s genes and metabolism, they discovered the specific enzymes and pathways it uses to degrade DBP into harmless compounds. This finding offers a practical biological solution for cleaning up contaminated agricultural soils, particularly in Xinjiang where plastic film mulching is widely used in cotton farming.

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