Research Keyword: culture media

Growth Characteristics of Polyporales Mushrooms for the Mycelial Mat Formation

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Researchers tested 64 types of mushroom strains to find which grows the strongest mycelial mats for making eco-friendly materials to replace plastic and other fossil fuel products. They discovered that Ganoderma lucidum, a medicinal mushroom, produces especially thick and strong mycelium that can be grown at large scales. By using the right nutrients like glycerol and skim milk, they were able to grow large sheets of mushroom material that could potentially be used for packaging, furniture, and other products instead of traditional plastics.

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Effect of Culture Media on the Yield and Protein Content of Pleurotus ostreatus (Jacq.) Kumm Mycelia

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This research optimized how to grow oyster mushroom mycelium in laboratory conditions to produce high-protein food ingredients. The scientists tested different nutrient sources, sugar types, and pH levels to find the best growing conditions. They discovered that adding urea and using amaranth flour as a base nearly doubled the protein content, making oyster mushroom mycelium a promising sustainable alternative to animal proteins.

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Cultivation and mycelium production from spore suspensions of desert truffles: prospective use as inoculum for host plants in arid zones

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Desert truffles are valuable edible fungi that grow in arid regions and have health benefits. This research successfully grew two desert truffle species (Terfezia boudieri and Tirmania nivea) in laboratory conditions using standard culture media. The study found that potato dextrose agar was the best medium for rapid growth, while both media supported good mycelial development. This breakthrough could enable large-scale production of truffle inoculum for reforestation programs in Morocco’s degraded desert areas.

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Cultivation and mycelium production from spore suspensions of desert truffles: prospective use as inoculum for host plants in arid zones

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Scientists successfully grew two types of desert truffles from Morocco in the laboratory using simple growth materials. By placing spores in potato dextrose agar or malt extract agar, they were able to produce substantial amounts of fungal threads (mycelium) within a week. This achievement is important because these truffles can now be used to help restore desert soils and grow edible mushrooms in arid regions, providing food and income for communities while fighting desertification.

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A novel, cheap and easy preparing selective medium for isolation of Pythium species

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Scientists developed an affordable and effective way to grow and study harmful Pythium fungi that cause crop diseases and infections in animals. The new FANS medium uses inexpensive antibiotics that are safer and cheaper than previous methods, making it accessible to researchers worldwide, especially in developing countries. This advancement helps researchers better understand and develop treatments for diseases caused by Pythium species in both agriculture and medicine.

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Phylogenetic placements and cultural characteristics of Tuber species isolated from ectomycorrhizas

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Researchers successfully grew truffle fungi in the laboratory by isolating them from the roots of fir trees in Japanese forests. They identified eight different truffle species or lineages and studied how they grew on nutrient agar plates, finding that while they shared basic characteristics like white filamentous colonies, they had many differences in growth rates and hyphal structure. The study shows that collecting truffles from tree roots can be an effective way to obtain pure cultures of these fungi when fruiting bodies cannot be found.

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Optimized protocol for culturing and extracting DNA from fungal isolates associated with brown spot needle blight in pine trees

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Scientists developed an optimized method for growing brown spot needle blight fungi from infected pine needles and extracting their DNA for research. They tested four different growth media and four DNA extraction techniques to find the best combination. Sabouraud dextrose medium combined with a CTAB-based extraction method containing polyvinylpyrrolidone worked best, producing high-quality DNA suitable for advanced genetic studies. This standardized approach will help researchers better understand this important forest disease.

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