CRISPR-Cas9 – mycolab.ai

The role of Micro-biome engineering in enhancing Food safety and quality

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Microbiome engineering uses advanced biotechnology to strategically modify helpful bacteria in food to make it safer and higher quality. By using tools like CRISPR gene editing and engineering beneficial probiotics, scientists can prevent food spoilage, reduce harmful bacteria, improve nutrition, and create better-tasting foods. These innovations could reduce reliance on synthetic preservatives and chemicals while addressing global food safety challenges and helping combat malnutrition.

The forced activation of asexual conidiation in Aspergillus niger simplifies bioproduction

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Researchers developed a new method to simplify the production of L-malic acid using genetically modified Aspergillus niger fungi. Instead of growing spores on solid plates—a time-consuming and labor-intensive process—they engineered the fungi to produce spores directly in liquid medium controlled by adding xylose. This simplified approach maintains the fungi’s ability to produce high levels of L-malic acid while significantly reducing costs and labor requirements for industrial production.

Edible mushrooms as emerging biofactories for natural therapeutics and oral biopharmaceutical delivery

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Mushrooms are emerging as natural medicine factories that can be genetically engineered to produce medications taken by mouth. Beyond their traditional use as health foods, scientists are now using advanced genetic techniques to program mushrooms to manufacture therapeutic proteins and vaccines. These engineered mushrooms can naturally package and protect these medications as they pass through the stomach, releasing them safely in the intestines for absorption. This approach offers a sustainable, affordable, and cold-chain-independent alternative to conventional injected medications.

Advances in Bioprocess Engineering for Optimising Chlorella vulgaris Fermentation: Biotechnological Innovations and Applications

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Chlorella vulgaris is a nutrient-rich microalga gaining popularity in health supplements, functional foods, and sustainable energy production. Scientists are using advanced genetic engineering techniques, special fermentation methods, and innovative bioreactor designs to increase the production of beneficial compounds like proteins and antioxidants. These improvements make Chlorella more valuable for creating health-promoting foods, medicines, and biofuels while keeping production costs low and environmentally sustainable.

A Comprehensive Review of the Diversity of Fungal Secondary Metabolites and Their Emerging Applications in Healthcare and Environment

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Fungi naturally produce complex chemical compounds called secondary metabolites that have powerful effects against diseases and pests. These include well-known medicines like penicillin and compounds that can fight cancer, reduce inflammation, and lower cholesterol. Scientists are now using advanced genetic and biotechnology techniques to increase production of these fungal compounds, making them more available and affordable for medical, agricultural, and environmental applications. This research shows how fungi could be important sources of new medicines and sustainable alternatives to synthetic chemicals.

High-Yield-Related Genes Participate in Mushroom Production

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Scientists have identified specific genes that control how mushrooms grow and produce fruit bodies. By using advanced gene-editing technology like CRISPR-Cas9, researchers can now increase mushroom yields by 20-65%, offering a faster and more efficient alternative to traditional breeding methods. This breakthrough could help meet the world’s growing demand for mushrooms while making farming more sustainable and economical for growers globally.

Recent developments of tools for genome and metabolome studies in basidiomycete fungi and their application to natural product research

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Mushrooms and related fungi in the basidiomycete group produce many useful medicines and agricultural chemicals. Scientists have traditionally struggled to study these fungi because they grow slowly and have complex genomes. Recent technological breakthroughs—including faster DNA sequencing and gene-editing tools—are now making it much easier to discover and understand the helpful compounds these fungi produce, potentially leading to new medicines.

Advances in the Degradation of Polycyclic Aromatic Hydrocarbons by Yeasts: A Review

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This review explores how yeasts, tiny single-celled fungi, can clean up environments contaminated with polycyclic aromatic hydrocarbons (PAHs) – harmful chemicals produced by car emissions, factories, and burning. These yeasts use special enzymes to break down these toxic compounds into less harmful substances, making them a promising natural solution for environmental cleanup. Scientists are also improving these yeasts through genetic engineering to make them even more effective at removing pollution.

Providing a toolbox for genomic engineering of Trichoderma aggressivum

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Scientists have created a set of tools to genetically engineer Trichoderma aggressivum, a fungus that causes green mold disease in cultivated mushrooms but can also be used beneficially. The study provides step-by-step methods for transforming this fungus using both traditional plasmid methods and modern CRISPR gene-editing technology. These tools will help researchers understand how the fungus works and potentially harness its beneficial properties for agriculture.

Strategy of employing plug-and-play vectors and LC–MS screening to facilitate the discovery of natural products using Aspergillus oryzae

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Researchers developed new tools to make it faster and easier to discover useful compounds from fungi. They created improved genetic vectors that allow scientists to insert multiple genes into Aspergillus oryzae more conveniently, and developed a quick screening method using mass spectrometry to identify successful transformants directly on culture plates. This approach saves about 10 days compared to traditional methods, significantly accelerating the discovery of new natural products with potential medical and agricultural applications.

Research Keyword: CRISPR-Cas9