Research Keyword: cell wall degradation

Establishment of a genetic transformation system for cordycipitoid fungus Cordyceps chanhua

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Scientists have successfully developed a method to genetically modify Cordyceps chanhua, an important medicinal mushroom used in traditional Chinese medicine for over 1,600 years. By optimizing how they prepare fungal cells and introduce foreign genes, they created a reliable genetic transformation system that can be used to study which genes produce beneficial compounds in this mushroom. This breakthrough will help researchers understand and potentially enhance the medicinal properties of C. chanhua.

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Optimization of Protoplast Preparation and Establishment of PEG-Mediated Genetic Transformation Method in Cordyceps cicadae

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Scientists successfully developed a method to genetically modify Cordyceps cicadae, a valuable medicinal fungus used in traditional Chinese medicine. By optimizing how to remove the fungus’s protective cell wall and introducing new genes using a technique called PEG-mediated transformation, researchers created a stable system for genetic manipulation. This breakthrough opens the door for improving medicinal compounds in this fungus and advancing its use in treating various health conditions.

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High temperature enhances the ability of Trichoderma asperellum to infect Pleurotus ostreatus mycelia

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Researchers discovered that summer heat makes oyster mushroom crops more vulnerable to green mold disease caused by a fungus called Trichoderma asperellum. When exposed to high temperatures (36°C), the pathogenic fungus becomes more aggressive by producing more spores, germinating faster, and generating molecules like hydrogen peroxide that damage the mushroom mycelia. Meanwhile, the oyster mushroom itself becomes more susceptible to infection at these higher temperatures, explaining why green mold outbreaks are so common during hot summer months in mushroom farms.

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Bacillus velezensis LMY3-5 for the biocontrol of soft rot in kiwifruit: antifungal action and underlying mechanisms

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Scientists discovered a beneficial bacteria called Bacillus velezensis that can effectively prevent soft rot disease in kiwifruit, which causes serious damage during storage. The bacteria works by producing natural antimicrobial compounds that damage the fungal pathogen’s cell walls and membranes, stopping it from growing. This biocontrol approach offers an environmentally friendly alternative to chemical fungicides, reducing pesticide residues while maintaining fruit quality and safety.

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The small GTPases FoRab5, FoRab7, and FoRab8 regulate vesicle transport to modulate vegetative development and pathogenicity in Fusarium oxysporum f. sp. conglutinans

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Scientists studied three proteins (Rab GTPases) in a fungal pathogen that causes cabbage wilt disease. These proteins act like traffic controllers, directing materials within fungal cells to support growth and disease spread. By removing these genes one at a time, researchers found that all three proteins are essential for the fungus to infect plants, produce spores, and survive stress conditions. This research could eventually help develop new ways to control this destructive crop disease.

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Improved Protoplast Production Protocol for Fungal Transformations Mediated by CRISPR/Cas9 in Botrytis cinerea Non-Sporulating Isolates

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Scientists have developed a better method to isolate protoplasts (fungal cells without cell walls) from non-sporulating varieties of gray mold fungus. By optimizing the incubation time, culture container, and enzyme used, they produced more viable protoplasts that can regenerate and be genetically modified. This advancement allows researchers to use CRISPR gene-editing technology to understand and potentially control gray mold, which causes significant crop losses worldwide.

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