Research Keyword: Bestkeeper

Selection of reliable reference genes in Colletotrichum scovillei during different growth stages, host interactions, and plant extract treatment for qRT-PCR

This research identifies the best internal control genes to use when measuring gene activity in a fungus that causes pepper disease. Scientists tested eight different reference genes under various conditions like fungal growth and pepper infection, plus when treating the fungus with plant extracts. Different genes worked best for different situations: one gene was best during fungal growth, another during infection, and a third when treated with plant extracts. These findings will help future studies better understand how this disease-causing fungus works and develop better control methods.

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Selection and validation of reference genes for RT-qPCR in ophiocordyceps sinensis under different experimental conditions

Scientists studied the Chinese caterpillar mushroom (Ophiocordyceps sinensis) to find the best genes to use as reference points when measuring other gene activity. They tested sixteen potential reference genes under various stress conditions and growth stages using a technique called RT-qPCR. They found that two specific genes (18S rRNA and beta-Tubulin) were the most reliable to use as standards, which will help researchers accurately study how this medicinal mushroom responds to different conditions.

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