Research Keyword: axenic seeds

A simple protocol for producing axenic seeds of Sorghum bicolor

This study presents a simple and inexpensive method for sterilizing sorghum seeds to remove all living microbes while keeping them viable for planting. The process involves soaking seeds in ethanol and bleach for short periods, followed by rinsing. Of 95 seeds tested, only 2 showed contamination, and over half successfully germinated, making this method practical for seed research.

Read More »

A simple protocol for producing axenic seeds of Sorghum bicolor

Researchers developed a simple method to sterilize sorghum seeds by treating them with ethanol and bleach, which removes contaminating microbes while keeping seeds alive and able to grow. This approach is inexpensive, requires no special equipment, and is useful for scientists studying how microbes interact with plants. The method successfully eliminated harmful microbes from 98% of seeds tested while maintaining a reasonable germination rate of 63%.

Read More »
Scroll to Top