Desert truffles are valuable edible fungi that grow in arid regions and have health benefits. This research successfully grew two desert truffle species (Terfezia boudieri and Tirmania nivea) in laboratory conditions using standard culture media. The study found that potato dextrose agar was the best medium for rapid growth, while both media supported good mycelial development. This breakthrough could enable large-scale production of truffle inoculum for reforestation programs in Morocco’s degraded desert areas.