Research Keyword: Agrobacterium-mediated transformation

Heterologous expression of the hypovirus CHV1-EP713 full-length cDNA in Botrytis cinerea: transformation with Agrobacterium tumefaciens and evaluation of changes in the fungal phenotype

Researchers successfully introduced a virus from chestnut blight fungus into gray mold fungus to reduce its ability to cause disease. The transformed fungus grew slower, produced fewer spores, and caused less damage to plants. This discovery suggests viruses could be used as natural biological control agents to protect crops from fungal diseases.

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Development of a molecular genetics and cell biology toolbox for the filamentous fungus Diplodia sapinea

Scientists have developed new tools to study a fungus called Diplodia sapinea that damages pine trees around the world. They created a method to genetically modify this fungus and tag its cell nuclei with a red fluorescent marker so they can track the infection process. They also developed a simple way to test infections using young pine seedlings in the laboratory instead of large greenhouse setups. Using these new tools together, researchers can now watch in real-time how the fungus grows inside infected pine plants, which will help develop better ways to protect forests.

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Construction of a heat-resistant strain of Lentinus edodes by fungal Hsp20 protein overexpression and genetic transformation

Scientists successfully created a heat-resistant version of shiitake mushrooms by adding extra copies of a heat-protection gene from button mushrooms. The modified mushrooms can survive higher temperatures and recover better after heat stress compared to regular shiitake strains. This genetic improvement could help shiitake farming expand to warmer regions and times of year, potentially increasing production worldwide.

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