Research Keyword: 16S rRNA

Pathogen Identification, Antagonistic Microbe Screening, and Biocontrol Strategies for Aconitum carmichaelii Root Rot

Aconitum carmichaelii is a traditional Chinese medicinal plant that has become increasingly prone to root rot disease due to continuous farming in Yunnan. Researchers identified multiple disease-causing pathogens and discovered that beneficial bacteria called Bacillus can both fight these pathogens and improve plant health. One particularly effective strain enhanced soil quality and increased the plant’s natural defenses, achieving over 50% disease control without chemical pesticides.

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The Effect of Pseudomonas putida on the Microbial Community in Casing Soil for the Cultivation of Morchella sextelata

Morel mushrooms are prized edible fungi, but growing them repeatedly in the same soil causes problems because toxic ethylene gas builds up and the soil microbiome becomes unbalanced. Scientists found that a beneficial soil bacterium called Pseudomonas putida can break down the ethylene precursor and improve the soil microbial community, making morels grow better and faster. This natural approach using microbial inoculation offers a practical solution to help farmers overcome these continuous cropping challenges.

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Biodegradation of BTEX by Bacteria Isolated From Soil Contaminated With Petroleum Sludge and Liquid and Solid Petrochemical Effluents

Scientists isolated bacteria from oil-contaminated soil that can effectively break down BTEX chemicals, which are toxic pollutants from petroleum products. Two bacterial strains, Arthrobacter pascens and Bacillus sp., proved most effective at degrading these harmful compounds, removing over 80% within 12 days. These findings suggest these bacteria could be used to clean up contaminated sites naturally and cost-effectively.

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The Expanding Truffle Environment: A Study of the Microbial Dynamics in the Old Productive Site and the New Tuber magnatum Picco Habitat

Researchers studied how microbial communities develop in white truffle forests, comparing an established productive forest with a nearby expanding area in Tuscany, Italy. Using DNA sequencing techniques, they tracked fungi and bacteria over two years and found that the expanding area had more diverse microbial communities while the established forest showed more stable, ectomycorrhizal-dominated communities. The white truffle fungus showed unique associations with specific bacterial types, particularly species that may enhance plant growth and nutrient acquisition. These findings suggest that proper forest management practices like selective vegetation cutting can support truffle production by creating favorable conditions for beneficial microbial communities.

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Metabarcoding Unveils Seasonal Soil Microbiota Shifts and Their Influence on Boletus edulis and Boletus reticulatus Mycelium in Quercus robur Stands

This research studied how soil bacteria and fungi change across fall months in oak forests in Spain and how these changes relate to the growth of valuable edible mushrooms (Boletus species). Scientists used DNA analysis to identify thousands of different microorganisms in the soil. They found that September had the most diverse microbial communities, October showed a decline, and November saw partial recovery. Importantly, Boletus mycelium showed different patterns of growth depending on the month and was associated with specific beneficial bacteria and fungi.

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The Expanding Truffle Environment: A Study of the Microbial Dynamics in the Old Productive Site and the New Tuber magnatum Picco Habitat

This study examines how microbial communities develop in white truffle forests over two years, comparing an established productive area with an adjacent newly expanding habitat. Researchers found that young expanding truffle areas have more diverse and dynamic microbial communities, while established forests show more stable but sensitive communities. The study identified specific bacteria that associate with truffle growth, providing insights into how forest management can help expand valuable white truffle habitats.

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Streptomyces-Based Bioformulation to Control Wilt of Morchella sextelata Caused by Pestalotiopsis trachicarpicola

Researchers discovered two beneficial bacteria called Streptomyces that can protect morel mushrooms from a harmful fungus causing wilt disease. When applied to morel cultivation fields, these bacteria not only prevented disease but also increased mushroom yields by about 30% compared to untreated fields. This natural biocontrol approach offers farmers an eco-friendly alternative to chemical treatments while boosting their harvests.

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Improving the Yield and Quality of Morchella spp. Using Agricultural Waste

Researchers tested whether recycling agricultural waste like spent mushroom compost, used tomato growing medium, and coconut shells could improve morel mushroom farming. Adding these waste materials significantly increased yields by three times or more while also improving the nutritional quality of the mushrooms. The waste materials changed the soil composition and beneficial microorganisms in ways that support better morel growth while reducing harmful fungi and environmental pollution.

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Insights into microbiome-triterpenoid correlation in Poria cocos via comparative analysis of sclerotial and soil microenvironments

Researchers discovered that the medicinal fungus Poria cocos creates its own specialized microbial environment in its underground structure that is closely linked to the production of pachymic acid, a compound with anti-cancer and immune-boosting properties. By comparing the microbes living in the fungus versus surrounding soil, they found specific beneficial bacteria and fungi that thrive in the fungus but are rare in soil. This discovery could help improve cultivation techniques to produce higher quality medicinal fungi with more therapeutic compounds.

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Microbial communities associated with the black morel Morchella sextelata cultivated in greenhouses

This study examined the tiny organisms living on and around cultivated black morel mushrooms grown in greenhouses in China. Researchers found that four main types of bacteria consistently live on morel fruiting bodies: Pedobacter, Pseudomonas, Stenotrophomonas, and Flavobacterium. The bacteria and fungi in the soil surrounding morels appear to be important for the mushrooms’ growth and development, with different microbial communities found on different parts of the mushroom.

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