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Whole-genome sequencing of Fusarium oxysporum K326-S isolated from tobacco

Summary

Scientists have sequenced the complete genetic blueprint of a fungus that causes root rot in tobacco plants. This fungus, Fusarium oxysporum K326-S, damages tobacco crops by causing roots to brown and wilt. The detailed genome map they created contains over 17,000 genes and will help farmers and researchers develop better strategies to prevent and control this destructive disease in the future.

Background

Root diseases caused by soil-borne pathogens increasingly threaten plant health and crop productivity. Fusarium oxysporum is a significant pathogenic fungus affecting various plant species including tobacco. Whole-genome sequencing of root-associated fungi provides critical insights for disease management and prevention strategies.

Objective

To present the complete whole-genome sequence of Fusarium oxysporum K326-S, a soil-borne pathogenic fungus isolated from tobacco roots. The study aims to provide valuable genomic resources for understanding this pathogen and supporting future disease prevention and control research.

Results

The assembled genome of F. oxysporum K326-S is 51,143,096 bp with 1,153 scaffolds and a maximum scaffold length of 1,078,849 bp. A total of 17,272 coding genes were predicted along with 310 tRNAs and 63 rRNAs. BUSCO analysis showed 98.9% genome completeness, indicating a highly complete fungal genome assembly.

Conclusion

The complete genome assembly of F. oxysporum K326-S expands available genomic resources for the F. oxysporum species complex. This genomic foundation provides essential data for future research on disease prevention and control mechanisms. The high-quality genome will support comparative genomics and pathogenicity studies.
  • Published in:Microbiology Resource Announcements,
  • Study Type:Genome Sequencing Study,
  • Source: PMID: 40928291, DOI: 10.1128/mra.00319-25
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