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Whole-Genome Sequence of a High-Temperature Edible Mushroom Pleurotus giganteus (Zhudugu)

Summary

Scientists have sequenced the complete genome of Pleurotus giganteus, an edible mushroom that uniquely thrives in high temperatures. This research provides valuable insights into how this mushroom can grow in warm conditions and break down wood materials efficiently. Impact on everyday life: – Enables development of better-growing mushroom varieties for warm climates – Helps improve commercial mushroom production methods – Could lead to more efficient processes for breaking down plant waste – May contribute to developing new nutritional and medicinal products – Supports sustainable agriculture through better understanding of mushroom cultivation

Background

Pleurotus giganteus is a culinary edible mushroom widely distributed and cultivated in China, Southeast Asia, and South Asia. It is known as ‘Zhudugu’ in China due to its organoleptic properties resembling pork stomach. Most sequenced wood-rotting edible mushrooms produce fruiting bodies at relatively low temperatures, but little information is known about high-temperature wood-rotting mushrooms.

Objective

To perform de novo sequencing and assembly of the genome of P. giganteus from a monokaryotic strain zhudugu2 using Illumina and Pac-Bio CLR sequencing technologies to better understand its high-temperature tolerance and other characteristics.

Results

The assembled genome consists of 40.00 Mb in 27 contigs with a contig N50 of 4.384 Mb. A total of 12,628 protein-coding genes were annotated. The genome showed high collinearity with P. ostreatus. Analysis identified 481 enzymes accounting for 514 carbohydrate-active enzymes terms, including 15 laccases and 10 class II peroxidases, revealing robust lignocellulose degradation capacity.

Conclusion

The high-quality genome sequence provides insights into the genetic basis of P. giganteus characteristics and will benefit genome-assisted breeding. The findings help understand the mushroom’s high-temperature tolerance and lignocellulose degradation capabilities. The genome information can be used for developing molecular markers for strain identification and breeding.
  • Published in:Frontiers in Microbiology,
  • Study Type:Genomic Analysis,
  • Source: 10.3389/fmicb.2022.941889
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