Untargeted Metabolite Profiling of Antimicrobial Compounds in the Brown Film of Lentinula edodes Mycelium via LC-MS/MS Analysis

This research examined how shiitake mushrooms develop a protective brown coating during cultivation that helps defend against harmful microorganisms. Scientists identified several natural antimicrobial compounds produced in this brown film that act as a defense system. This discovery helps explain how mushrooms protect themselves during farming and could lead to new natural antimicrobial products. Impacts on everyday life: – Improved understanding of mushroom farming could lead to better cultivation methods – Natural antimicrobial compounds discovered could be developed into new preservatives or medicines – Better mushroom protection methods could increase crop yields and reduce food costs – Understanding natural defense mechanisms could help develop more sustainable farming practices – Discovery of new bioactive compounds could lead to novel therapeutic applications