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Transcriptomic Profiling of Thermotolerant Sarcomyxa edulis PQ650759 Reveals the Key Genes and Pathways During Fruiting Body Formation

Summary

Researchers studied how a special strain of Sarcomyxa edulis (a tasty edible mushroom from Northeast China) develops fruiting bodies by analyzing which genes are turned on and off during this process. By comparing immature mycelium with developing fruiting bodies, they identified key genes responsible for cell division, DNA repair, and energy metabolism that control fruiting body formation. This knowledge can help mushroom farmers improve yield and quality through better understanding of how mushrooms grow. The findings provide a foundation for developing better cultivation techniques and selecting superior mushroom strains for commercial production.

Background

Sarcomyxa edulis is a low-temperature edible mushroom native to Northeast China with rich nutritional and medicinal properties. The species can undergo explosive fruiting and unified harvesting, making it suitable for factory production. However, the molecular mechanisms underlying fruiting body development in S. edulis remain poorly understood.

Objective

This study employed transcriptome analysis to compare post-ripening mycelium (NPM) and primordial fruiting bodies (PRMs) of the thermostable S. edulis strain PQ650759, which uniquely forms primordia under constant temperature conditions. The goal was to identify key genes and metabolic pathways involved in fruiting body formation.

Results

Analysis revealed 2176 upregulated and 2666 downregulated genes between PRM and NPM stages. Key findings included enrichment in cell cycle regulation, DNA replication, and carbohydrate metabolism. Ten candidate genes including SKP1, MRE11, and GPI were identified as crucial regulators. qPCR validation confirmed RNA-seq results with consistent expression trends.

Conclusion

The study identifies conserved and novel genetic drivers including SKP1 and MRE11 that underpin primordium formation under constant-temperature conditions. Findings advance mechanistic understanding of fungal developmental biology and provide actionable targets for optimizing industrial-scale cultivation and strain optimization of this economically valuable species.
  • Published in:Journal of Fungi,
  • Study Type:Transcriptomic Analysis Study,
  • Source: PMID: 40985412, PMCID: PMC12295817, DOI: 10.3390/jof11070484
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