Transcriptomic and Non-Targeted Metabolomic Analyses Reveal the Flavonoid Biosynthesis Pathway in Auricularia cornea
- Author: mycolabadmin
- 2022-04-04
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Summary
This research reveals how mushrooms can produce beneficial compounds called flavonoids, which are typically found in plants. The scientists discovered that adding plant extract to mushroom growth medium increased flavonoid production and mapped out the genetic pathway responsible for making these compounds. This has important implications for human health and nutrition.
Impacts on everyday life:
• Could lead to more nutritious mushroom products with enhanced health benefits
• May enable development of new natural supplements with higher antioxidant content
• Provides potential new ways to produce beneficial plant compounds using fungi
• Could help make medicinal mushrooms more effective for treating various conditions
• May lead to more sustainable production of beneficial compounds typically sourced from plants
Background
Flavonoids are important secondary metabolites recognized for their antioxidant and anticancer roles in clinical applications. While abundant in plants, little is known about the molecular basis of flavonoid biosynthesis in fungi. Auricularia cornea is widely cultivated in China and has long been used as a dietary supplement and medicinal mushroom, with properties primarily associated with polysaccharides and flavonoids in their fruiting bodies.
Objective
The study had two main objectives: 1) To investigate if leachate from Caragana korshinskii used as fermentation medium could increase the total flavonoid content of A. cornea, and 2) To elucidate the flavonoid biosynthesis pathway in A. cornea through transcriptomic and metabolomic analyses.
Results
The addition of C. korshinskii leachate (75 g/L) increased total flavonoid content by 23.6% compared to control medium. Nine metabolites were identified in the flavonoid biosynthesis pathways, specifically in phenylpropanoid, coumarin, and isoflavonoid biosynthesis pathways. The study identified four homologous genes encoding phenylalanine ammonia lyase (PAL), three encoding polyphenol oxidase (PPO), and two encoding chalcone isomerase (CHI).
Conclusion
The study demonstrated that C. korshinskii leachate in fermentation medium successfully increased flavonoid content in A. cornea. The research mapped important metabolites in the phenylpropanoid, coumarin and isoflavonoid biosynthesis pathways and identified key genes encoding enzymes involved in flavonoid biosynthesis. These findings provide new insights for studying flavonoid production regulation in edible fungi.
- Published in:Molecules,
- Study Type:Laboratory Research,
- Source: 10.3390/molecules27072334