Transcriptome of Different Fruiting Stages in the Cultivated Mushroom Cyclocybe aegerita Suggests a Complex Regulation of Fruiting and Reveals Enzymes Putatively Involved in Fungal Oxylipin Biosynthesis
- Author: mycolabadmin
- 2021-05-04
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Summary
This research examined how genes are activated during mushroom development in the commercially cultivated species Cyclocybe aegerita, focusing on understanding how mushrooms produce their characteristic aromas and develop their structure. The study revealed complex genetic regulation during mushroom formation and identified key enzymes involved in producing mushroom scent compounds.
Impacts on everyday life:
– Improved understanding of mushroom cultivation and development could lead to better farming techniques
– Knowledge of aroma compound production could help develop enhanced mushroom flavors for food applications
– Understanding fungal development processes aids in optimizing commercial mushroom production
– Insights into volatile compound production could lead to new natural flavor compounds
– Better grasp of mushroom biology supports sustainable food production methods
Background
Cyclocybe aegerita (syn. Agrocybe aegerita) is a commercially cultivated mushroom. Its archetypal agaric morphology and ability to undergo its whole life cycle under laboratory conditions makes it well-suited for studying fruiting body development. The formation of fruiting bodies in Basidiomycota is one of the most complex developmental processes in the fungal life cycle, involving various morphological changes and metabolic processes that are still poorly understood.
Objective
To elucidate the biosynthesis of fungal volatiles and analyze alterations in the transcriptome during different developmental stages of C. aegerita, combining changes in gene expression with variations in the volatile profile during fruiting stages.
Results
The study identified differential expression of genes involved in fungal fruiting body formation showing distinct transcriptional patterns correlating with developmental stages. By combining transcriptome and volatilome data, researchers identified enzymes putatively involved in C8 oxylipin biosynthesis including lipoxygenases, dioxygenases, hydroperoxide lyases, alcohol dehydrogenases and ene-reductases. The mycelium was identified as the main source for sesquiterpenes predominant during sporulation, while changes in the C8 profile detected in late stages were attributed to enzyme activity in the fruiting bodies.
Conclusion
The combination of volatilome and transcriptome data revealed promising candidates for both functional genetics-based analysis of fruiting-related genes and enzyme characterization studies to further understand fungal C8 oxylipin biosynthesis. The study demonstrated complex regulation of fruiting body development and volatile compound production in different fungal tissues.
- Published in:BMC Genomics,
- Study Type:Transcriptome Analysis,
- Source: 10.1186/s12864-021-07648-5