Transcriptome Data Reveal Conserved Patterns of Fruiting Body Development and Response to Heat Stress in the Mushroom-Forming Fungus Flammulina filiformis
- Author: mycolabadmin
- 2020-10-16
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Summary
This research examined how winter mushrooms (Flammulina filiformis) develop and respond to heat stress at the genetic level. The study revealed important genes that control mushroom formation and identified mechanisms that allow some mushroom strains to better tolerate warm temperatures. This knowledge has practical implications for mushroom cultivation and broader significance for understanding how complex organisms develop.
Impacts on everyday life:
• Could lead to improved mushroom varieties that grow better in warmer conditions
• May help reduce energy costs in commercial mushroom production by requiring less cooling
• Provides insights that could help maintain mushroom supplies despite climate change
• Advances understanding of how organisms develop complex structures
• Could contribute to more efficient and sustainable mushroom farming methods
Background
Mushroom-forming fungi are complex multicellular organisms that form the basis of a large industry, yet understanding of mushroom development mechanisms and stress responses remains limited. The winter mushroom (Flammulina filiformis) is commercially cultivated at large scale in East Asia and prefers low temperatures.
Objective
This study aimed to investigate fruiting body development in F. filiformis by comparing transcriptomes across 4 developmental stages, identify conserved genes involved in fruiting body development through comparison with a 200-genome dataset, and examine heat stress responses between heat sensitive and resistant strains.
Results
The study revealed widely conserved genes involved in primordium development of F. filiformis, many originating before the emergence of Agaricomycetes, indicating co-option for complex multicellularity during evolution. Several fruiting-specific genes were identified, including those with conserved stipe-specific expression patterns and others related to sexual development, water absorption, basidium formation and sporulation. Heat stress induced more genes in the heat resistant strain (M1) than the sensitive strain (XR), particularly hsp70, hsp90 and fes1 genes that may facilitate heat stress adjustment during early fruiting body development.
Conclusion
The study expanded knowledge of fruiting body development and heat stress response in mushroom-forming fungi through transcriptomic analysis of F. filiformis. Key genes showing conserved expression during development were identified, as well as heat defense mechanisms in the heat tolerant strain. The findings provide candidates for experimental follow-up studies to better understand specific gene roles in mushroom development.
- Published in:PLoS One,
- Study Type:Transcriptome Analysis,
- Source: 10.1371/journal.pone.0239890